Effects of emulsifier charge and concentration on pancreatic lipolysis. 1. in the absence of bile salts
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
- Sofia University St. Kliment Ohridski
- Unilever Research and Development
An in vitro study is performed with sunflower oil-in-water emulsions to clarify the effects of type of used emulsifier, its concentration, and reaction time on the degree of oil lipolysis, α. Anionic, nonionic, and cationic surfactants are studied as emulsifiers. For all systems, three regions are observed when surfactant concentration is scaled with the critical micelle concentration, C S/cmc: (1) At C S < cmc, α ≈ 0.5 after 30 min and increases up to 0.9 after 4 h. (2) At C S ≈ 3 × cmc, α ≈ 0.15 after 30 min and increases steeply up to 0.9 after 2 h for the cationic and nonionic surfactants, whereas it remains around 0.2 for the anionic surfactants. (3) At C S above certain threshold value, α = 0 for all studied surfactants, for reaction time up to 8 h. Additional experiments show that the lipase hydrolyzes molecularly soluble substrate (tributirin) at C S ≫ cmc, which is a proof that these surfactants do not denature or block the enzyme active center. Thus, we conclude that the mechanism of enzyme inhibition by these surfactants is the formation of a dense adsorption layer on an oil drop surface, which displaces the lipase from direct contact with the triglycerides.
|Number of pages||13|
|Publication status||Published - 29 May 2012|