Effect of triclosan or a phenolic farm disinfectant upon the selection of antibiotic resistant Salmonella enterica

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Effect of triclosan or a phenolic farm disinfectant upon the selection of antibiotic resistant Salmonella enterica. / Randall, LP; Cooles, SW; Piddock, Laura; Woodward, MJ.

In: Journal of Antimicrobial Chemotherapy, Vol. 54, 28.07.2004, p. 621-627.

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@article{abe49a164a03420c8353ab51ba2913a6,
title = "Effect of triclosan or a phenolic farm disinfectant upon the selection of antibiotic resistant Salmonella enterica",
abstract = "OBJECTIVE: To determine the effect of growth of five strains of Salmonella enterica and their isogenic multiply antibiotic-resistant (MAR) derivatives with a phenolic farm disinfectant or triclosan (biocides) upon the frequency of mutation to resistance to antibiotics or cyclohexane. METHODS: Strains were grown in broth with or without the biocides and then spread on to agar containing ampicillin, ciprofloxacin or tetracycline each at 4x MIC or agar overlaid with cyclohexane. Incubation was for 24 and 48 h and the frequency of mutation to resistance was calculated for strains with and without prior growth with the biocides. MICs were determined and the presence of mutations in the acrR and marR regions was determined by sequencing and the presence of mutations in gyrA by light-cycler analysis, for a selection of the mutants that arose. RESULTS: The mean frequency of mutation to antibiotic or cyclohexane resistance was increased approximately 10- to 100-fold by prior growth with the phenolic disinfectant or triclosan. The increases were statistically significant for all antibiotics and cyclohexane following exposure to the phenolic disinfectant (P 1 mg/L ciprofloxacin arose only from strains that were MAR. Reduced susceptibility to ciprofloxacin (at 4x MIC for parent strains) alone was associated with mutations in gyrA. MAR mutants did not contain mutations in the acrR or marR region. CONCLUSIONS: These data renew fears that the use of biocides may lead to an increased selective pressure towards antibiotic resistance.",
keywords = "cyclohexane, gyrA, MAR, ciprofloxacin, efflux",
author = "LP Randall and SW Cooles and Laura Piddock and MJ Woodward",
year = "2004",
month = jul
day = "28",
doi = "10.1093/jac/dkh376",
language = "English",
volume = "54",
pages = "621--627",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Effect of triclosan or a phenolic farm disinfectant upon the selection of antibiotic resistant Salmonella enterica

AU - Randall, LP

AU - Cooles, SW

AU - Piddock, Laura

AU - Woodward, MJ

PY - 2004/7/28

Y1 - 2004/7/28

N2 - OBJECTIVE: To determine the effect of growth of five strains of Salmonella enterica and their isogenic multiply antibiotic-resistant (MAR) derivatives with a phenolic farm disinfectant or triclosan (biocides) upon the frequency of mutation to resistance to antibiotics or cyclohexane. METHODS: Strains were grown in broth with or without the biocides and then spread on to agar containing ampicillin, ciprofloxacin or tetracycline each at 4x MIC or agar overlaid with cyclohexane. Incubation was for 24 and 48 h and the frequency of mutation to resistance was calculated for strains with and without prior growth with the biocides. MICs were determined and the presence of mutations in the acrR and marR regions was determined by sequencing and the presence of mutations in gyrA by light-cycler analysis, for a selection of the mutants that arose. RESULTS: The mean frequency of mutation to antibiotic or cyclohexane resistance was increased approximately 10- to 100-fold by prior growth with the phenolic disinfectant or triclosan. The increases were statistically significant for all antibiotics and cyclohexane following exposure to the phenolic disinfectant (P 1 mg/L ciprofloxacin arose only from strains that were MAR. Reduced susceptibility to ciprofloxacin (at 4x MIC for parent strains) alone was associated with mutations in gyrA. MAR mutants did not contain mutations in the acrR or marR region. CONCLUSIONS: These data renew fears that the use of biocides may lead to an increased selective pressure towards antibiotic resistance.

AB - OBJECTIVE: To determine the effect of growth of five strains of Salmonella enterica and their isogenic multiply antibiotic-resistant (MAR) derivatives with a phenolic farm disinfectant or triclosan (biocides) upon the frequency of mutation to resistance to antibiotics or cyclohexane. METHODS: Strains were grown in broth with or without the biocides and then spread on to agar containing ampicillin, ciprofloxacin or tetracycline each at 4x MIC or agar overlaid with cyclohexane. Incubation was for 24 and 48 h and the frequency of mutation to resistance was calculated for strains with and without prior growth with the biocides. MICs were determined and the presence of mutations in the acrR and marR regions was determined by sequencing and the presence of mutations in gyrA by light-cycler analysis, for a selection of the mutants that arose. RESULTS: The mean frequency of mutation to antibiotic or cyclohexane resistance was increased approximately 10- to 100-fold by prior growth with the phenolic disinfectant or triclosan. The increases were statistically significant for all antibiotics and cyclohexane following exposure to the phenolic disinfectant (P 1 mg/L ciprofloxacin arose only from strains that were MAR. Reduced susceptibility to ciprofloxacin (at 4x MIC for parent strains) alone was associated with mutations in gyrA. MAR mutants did not contain mutations in the acrR or marR region. CONCLUSIONS: These data renew fears that the use of biocides may lead to an increased selective pressure towards antibiotic resistance.

KW - cyclohexane

KW - gyrA

KW - MAR

KW - ciprofloxacin

KW - efflux

UR - http://www.scopus.com/inward/record.url?scp=4644258833&partnerID=8YFLogxK

U2 - 10.1093/jac/dkh376

DO - 10.1093/jac/dkh376

M3 - Article

C2 - 15269199

VL - 54

SP - 621

EP - 627

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

ER -