Abstract
The present study was undertaken to examine the role of the exercise-induced stress hormone response on the regulation of type 1 and type 2 T lymphocyte intracellular cytokine production. Subjects performed 2.5 h of cycling exercise at 65% maximal O-2 uptake while ingesting a 6.4% carbohydrate (CHO) solution, 12.8% CHO solution, or a placebo. Peripheral whole blood samples were stimulated and stained for T lymphocyte surface antigens (CD4 and CD8). Cells were then permeabilized, stained for intracellular cytokines, and analyzed using flow cytometry. Exercise resulted in a decrease (P <0.05) in the number and percentage of IFN-γ positive CD4(+) and CD8(+) T lymphocytes. These stimulated cells produced less IFN-γ immediately postexercise (P <0.05) and 2-h postexercise (P <0.05) compared with preexercise. However, CHO ingestion, which attenuated the exercise-induced stress hormone response compared with placebo (P <0.05), prevented both the decrease in the number and percentage of IFN-gamma-positive CD4(+) and CD8(+) T lymphocytes and the suppression of IFN-gamma production from stimulated CD4(+) and CD8(+) T lymphocytes. There was no effect of exercise on the number of, or cytokine production from, IL-4-positive CD4(+) or CD8(+) T lymphocytes. These data provide support for the role of exercise-induced elevations in stress hormones in the regulation of type 1 T lymphocyte cytokine production and distribution.
Original language | English |
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Pages (from-to) | 565-571 |
Number of pages | 7 |
Journal | Journal of Applied Physiology |
Volume | 98 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Oct 2004 |
Keywords
- cytokines
- immune
- cycling
- T lymphocytes