Effect of fluoroquinolone exposure on teh proteome of Salmonella enterica serovar Typhimurium

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Effect of fluoroquinolone exposure on teh proteome of Salmonella enterica serovar Typhimurium. / Coldham, NG; Randall, LP; Woodward, MJ; Piddock, Laura.

In: Journal of Antimicrobial Chemotherapy, Vol. 58, 20.10.2006, p. 1145-53.

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@article{bcacda98cbaa4d6c8a122630a2a83e29,
title = "Effect of fluoroquinolone exposure on teh proteome of Salmonella enterica serovar Typhimurium",
abstract = "OBJECTIVES: The physiological response of Salmonella enterica serovar Typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods. METHODS: Proteomes were prepared from strain SL1344 following treatment of broth cultures with ciprofloxacin (0.03 and 0.008 mg/L; 2x and 0.5x MIC) and enrofloxacin (0.03 mg/L) and from a multiple antibiotic resistant (MAR) mutant. Protein expression was determined by two-dimensional HPLC-MS(n) and also after exposure to ciprofloxacin by two-dimensional gel electrophoresis (2D-GE). RESULTS: The number of proteins (mean +/- SD) detected by 2D-GE derived from control cultures of the wild-type strain was significantly (P <0.05) reduced from 296 +/- 77 to 153 +/- 36 following treatment with ciprofloxacin (0.03 mg/L). Raised expression (P <0.05) of 17 proteins was also detected, and increases of up to 8-fold (P <0.0001) were observed for subunits of F1F0-ATP synthase, TolC and Imp. Analysis by two-dimensional HPLC-MS(n) provided higher proteome coverage with 787 +/- 50 proteins detected, which was reduced (P <0.005) to 560 +/- 14 by ciprofloxacin (0.03 mg/L). Increased expression of 43 proteins was observed which included those detected by 2D-GE and additionally the efflux pump protein AcrB. The basal expression of the AcrAB/TolC efflux pump was elevated in the MAR mutant compared with the untreated wild-type and augmented following treatment with ciprofloxacin (0.03 mg/L). F1F0-ATP synthase and Imp were only elevated in the mutant when treated with ciprofloxacin. CONCLUSIONS: These studies suggest that increased expression of AcrAB/TolC was associated with resistance while other increases, such as in F1F0-ATP synthase and Imp, were a response to fluoroquinolone.",
keywords = "fluoroquinolones, S. enterica, proteomics",
author = "NG Coldham and LP Randall and MJ Woodward and Laura Piddock",
year = "2006",
month = oct,
day = "20",
doi = "10.1093/jac/dkl413",
language = "English",
volume = "58",
pages = "1145--53",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Effect of fluoroquinolone exposure on teh proteome of Salmonella enterica serovar Typhimurium

AU - Coldham, NG

AU - Randall, LP

AU - Woodward, MJ

AU - Piddock, Laura

PY - 2006/10/20

Y1 - 2006/10/20

N2 - OBJECTIVES: The physiological response of Salmonella enterica serovar Typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods. METHODS: Proteomes were prepared from strain SL1344 following treatment of broth cultures with ciprofloxacin (0.03 and 0.008 mg/L; 2x and 0.5x MIC) and enrofloxacin (0.03 mg/L) and from a multiple antibiotic resistant (MAR) mutant. Protein expression was determined by two-dimensional HPLC-MS(n) and also after exposure to ciprofloxacin by two-dimensional gel electrophoresis (2D-GE). RESULTS: The number of proteins (mean +/- SD) detected by 2D-GE derived from control cultures of the wild-type strain was significantly (P <0.05) reduced from 296 +/- 77 to 153 +/- 36 following treatment with ciprofloxacin (0.03 mg/L). Raised expression (P <0.05) of 17 proteins was also detected, and increases of up to 8-fold (P <0.0001) were observed for subunits of F1F0-ATP synthase, TolC and Imp. Analysis by two-dimensional HPLC-MS(n) provided higher proteome coverage with 787 +/- 50 proteins detected, which was reduced (P <0.005) to 560 +/- 14 by ciprofloxacin (0.03 mg/L). Increased expression of 43 proteins was observed which included those detected by 2D-GE and additionally the efflux pump protein AcrB. The basal expression of the AcrAB/TolC efflux pump was elevated in the MAR mutant compared with the untreated wild-type and augmented following treatment with ciprofloxacin (0.03 mg/L). F1F0-ATP synthase and Imp were only elevated in the mutant when treated with ciprofloxacin. CONCLUSIONS: These studies suggest that increased expression of AcrAB/TolC was associated with resistance while other increases, such as in F1F0-ATP synthase and Imp, were a response to fluoroquinolone.

AB - OBJECTIVES: The physiological response of Salmonella enterica serovar Typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods. METHODS: Proteomes were prepared from strain SL1344 following treatment of broth cultures with ciprofloxacin (0.03 and 0.008 mg/L; 2x and 0.5x MIC) and enrofloxacin (0.03 mg/L) and from a multiple antibiotic resistant (MAR) mutant. Protein expression was determined by two-dimensional HPLC-MS(n) and also after exposure to ciprofloxacin by two-dimensional gel electrophoresis (2D-GE). RESULTS: The number of proteins (mean +/- SD) detected by 2D-GE derived from control cultures of the wild-type strain was significantly (P <0.05) reduced from 296 +/- 77 to 153 +/- 36 following treatment with ciprofloxacin (0.03 mg/L). Raised expression (P <0.05) of 17 proteins was also detected, and increases of up to 8-fold (P <0.0001) were observed for subunits of F1F0-ATP synthase, TolC and Imp. Analysis by two-dimensional HPLC-MS(n) provided higher proteome coverage with 787 +/- 50 proteins detected, which was reduced (P <0.005) to 560 +/- 14 by ciprofloxacin (0.03 mg/L). Increased expression of 43 proteins was observed which included those detected by 2D-GE and additionally the efflux pump protein AcrB. The basal expression of the AcrAB/TolC efflux pump was elevated in the MAR mutant compared with the untreated wild-type and augmented following treatment with ciprofloxacin (0.03 mg/L). F1F0-ATP synthase and Imp were only elevated in the mutant when treated with ciprofloxacin. CONCLUSIONS: These studies suggest that increased expression of AcrAB/TolC was associated with resistance while other increases, such as in F1F0-ATP synthase and Imp, were a response to fluoroquinolone.

KW - fluoroquinolones

KW - S. enterica

KW - proteomics

U2 - 10.1093/jac/dkl413

DO - 10.1093/jac/dkl413

M3 - Article

C2 - 17062612

VL - 58

SP - 1145

EP - 1153

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

ER -