Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells

Research output: Contribution to journalArticlepeer-review

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Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells. / Petridis, Xenos; Beems, Bas Pieter; Tomson, Phillip; Scheven, Ben; Giepmans, Ben; Kuipers, Jeroen; van der Sluis, Luc; Harmsen, Martin C.

In: Tissue Engineering Part A, Vol. 25, No. 15-16, 05.08.2019, p. 1104-1115.

Research output: Contribution to journalArticlepeer-review

Harvard

Petridis, X, Beems, BP, Tomson, P, Scheven, B, Giepmans, B, Kuipers, J, van der Sluis, L & Harmsen, MC 2019, 'Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells', Tissue Engineering Part A, vol. 25, no. 15-16, pp. 1104-1115. https://doi.org/10.1089/ten.TEA.2018.0192

APA

Petridis, X., Beems, B. P., Tomson, P., Scheven, B., Giepmans, B., Kuipers, J., van der Sluis, L., & Harmsen, M. C. (2019). Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells. Tissue Engineering Part A, 25(15-16), 1104-1115. https://doi.org/10.1089/ten.TEA.2018.0192

Vancouver

Author

Petridis, Xenos ; Beems, Bas Pieter ; Tomson, Phillip ; Scheven, Ben ; Giepmans, Ben ; Kuipers, Jeroen ; van der Sluis, Luc ; Harmsen, Martin C. / Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells. In: Tissue Engineering Part A. 2019 ; Vol. 25, No. 15-16. pp. 1104-1115.

Bibtex

@article{c932eaedcb8e4ce29a7331f0d2d0811c,
title = "Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells",
abstract = "In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. Here, we show that low concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared to DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMCs-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions upon a poorly organized collagen network was the prominent feature of UC-MSCs cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSCs cultures. The ability of dEMCs to induce mineralization varies between different human MSCs types in terms of total mineral formation and its architecture. Mineral formation by UC-MSCs exposed to low concentration dEMCs proved to be the most efficient and therefore could be considered as a promising combination for mineralized tissue engineering.",
keywords = "dental pulp, dentin matrix, mesenchymal stromal cells, mineralization, tissue engineering, umbilical cord",
author = "Xenos Petridis and Beems, {Bas Pieter} and Phillip Tomson and Ben Scheven and Ben Giepmans and Jeroen Kuipers and {van der Sluis}, Luc and Harmsen, {Martin C}",
year = "2019",
month = aug,
day = "5",
doi = "10.1089/ten.TEA.2018.0192",
language = "English",
volume = "25",
pages = "1104--1115",
journal = "Tissue Engineering Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert",
number = "15-16",

}

RIS

TY - JOUR

T1 - Effect of dentin matrix components on the mineralization of human mesenchymal stromal cells

AU - Petridis, Xenos

AU - Beems, Bas Pieter

AU - Tomson, Phillip

AU - Scheven, Ben

AU - Giepmans, Ben

AU - Kuipers, Jeroen

AU - van der Sluis, Luc

AU - Harmsen, Martin C

PY - 2019/8/5

Y1 - 2019/8/5

N2 - In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. Here, we show that low concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared to DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMCs-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions upon a poorly organized collagen network was the prominent feature of UC-MSCs cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSCs cultures. The ability of dEMCs to induce mineralization varies between different human MSCs types in terms of total mineral formation and its architecture. Mineral formation by UC-MSCs exposed to low concentration dEMCs proved to be the most efficient and therefore could be considered as a promising combination for mineralized tissue engineering.

AB - In teeth with an injured pulp, dentin matrix orchestrates hard tissue repair through the release of dentin extracellular matrix components (dEMCs). dEMCs regulate the differentiation of resident mesenchymal stromal cells (MSCs), thereby affecting mineral deposition. Here, we show that low concentration solubilized dEMCs in osteogenic cultures of human umbilical cord mesenchymal stromal cells (UC-MSCs) and dental pulp stromal cells (DPSCs) enhanced mineral deposition, while adipose stromal cells (ASCs) were barely affected. Interestingly, UC-MSCs displayed significantly greater hydroxyapatite formation compared to DPSCs. UC-MSCs and DPSCs showed a dose-dependent viability and proliferation, whereas proliferation of ASCs remained unaffected. Qualitative analysis of the dEMCs-supplemented osteogenic cultures through scanning electron microscopy demonstrated differences in the architecture of the deposited mineralized structures. Large-sized mineral accretions upon a poorly organized collagen network was the prominent feature of UC-MSCs cultures, while mineral nodules interspersed throughout a collagen mesh were observed in the respective DPSCs cultures. The ability of dEMCs to induce mineralization varies between different human MSCs types in terms of total mineral formation and its architecture. Mineral formation by UC-MSCs exposed to low concentration dEMCs proved to be the most efficient and therefore could be considered as a promising combination for mineralized tissue engineering.

KW - dental pulp

KW - dentin matrix

KW - mesenchymal stromal cells

KW - mineralization

KW - tissue engineering

KW - umbilical cord

U2 - 10.1089/ten.TEA.2018.0192

DO - 10.1089/ten.TEA.2018.0192

M3 - Article

C2 - 30444193

VL - 25

SP - 1104

EP - 1115

JO - Tissue Engineering Part A

JF - Tissue Engineering Part A

SN - 1937-3341

IS - 15-16

ER -