E. coli Fis protein insulates the cbpA gene from uncontrolled transcription

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E. coli Fis protein insulates the cbpA gene from uncontrolled transcription. / Chintakayala, Kiran; Singh, Shivani S.; Rossiter, Amanda E.; Shahapure, Rajesh; Dame, Remus T.; Grainger, David C.

In: PLoS Genetics, Vol. 9, No. 1, e1003152, 17.01.2013.

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@article{6e3693aeb7564082a88605f8f43ffafd,
title = "E. coli Fis protein insulates the cbpA gene from uncontrolled transcription",
abstract = "The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase. Using genetics, biochemistry, and genomics, we have examined regulation of, and DNA binding by, CbpA. We show that Fis, the dominant growth-phase nucleoid protein, prevents CbpA expression in growing cells. Regulation by Fis involves an unusual “insulation” mechanism. Thus, Fis protects cbpA from the effects of a distal promoter, located in an adjacent gene. In stationary phase, when Fis levels are low, CbpA binds the E. coli chromosome with a preference for the intrinsically curved Ter macrodomain. Disruption of the cbpA gene prompts dramatic changes in DNA topology. Thus, our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.",
keywords = "Bacterial genomics, Cell binding, Cell fusion, Chromosomes, DNA sequences, DNA transcription, DNA-binding proteins, RNA polymerase",
author = "Kiran Chintakayala and Singh, {Shivani S.} and Rossiter, {Amanda E.} and Rajesh Shahapure and Dame, {Remus T.} and Grainger, {David C.}",
year = "2013",
month = jan,
day = "17",
doi = "10.1371/journal.pgen.1003152",
language = "English",
volume = "9",
journal = "PLoS Genetics",
issn = "1553-7390",
publisher = "Public Library of Science (PLOS)",
number = "1",

}

RIS

TY - JOUR

T1 - E. coli Fis protein insulates the cbpA gene from uncontrolled transcription

AU - Chintakayala, Kiran

AU - Singh, Shivani S.

AU - Rossiter, Amanda E.

AU - Shahapure, Rajesh

AU - Dame, Remus T.

AU - Grainger, David C.

PY - 2013/1/17

Y1 - 2013/1/17

N2 - The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase. Using genetics, biochemistry, and genomics, we have examined regulation of, and DNA binding by, CbpA. We show that Fis, the dominant growth-phase nucleoid protein, prevents CbpA expression in growing cells. Regulation by Fis involves an unusual “insulation” mechanism. Thus, Fis protects cbpA from the effects of a distal promoter, located in an adjacent gene. In stationary phase, when Fis levels are low, CbpA binds the E. coli chromosome with a preference for the intrinsically curved Ter macrodomain. Disruption of the cbpA gene prompts dramatic changes in DNA topology. Thus, our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.

AB - The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase. Using genetics, biochemistry, and genomics, we have examined regulation of, and DNA binding by, CbpA. We show that Fis, the dominant growth-phase nucleoid protein, prevents CbpA expression in growing cells. Regulation by Fis involves an unusual “insulation” mechanism. Thus, Fis protects cbpA from the effects of a distal promoter, located in an adjacent gene. In stationary phase, when Fis levels are low, CbpA binds the E. coli chromosome with a preference for the intrinsically curved Ter macrodomain. Disruption of the cbpA gene prompts dramatic changes in DNA topology. Thus, our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.

KW - Bacterial genomics

KW - Cell binding

KW - Cell fusion

KW - Chromosomes

KW - DNA sequences

KW - DNA transcription

KW - DNA-binding proteins

KW - RNA polymerase

U2 - 10.1371/journal.pgen.1003152

DO - 10.1371/journal.pgen.1003152

M3 - Article

VL - 9

JO - PLoS Genetics

JF - PLoS Genetics

SN - 1553-7390

IS - 1

M1 - e1003152

ER -