Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation

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@article{e1ba6b63c0154138994318ea44ecfccc,
title = "Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation",
abstract = "Metazoan development involves the successive activation and silencing of specific gene expression programs and is driven by tissue-specific transcription factors programming the chromatin landscape. To understand how this process executes an entire developmental pathway, we generated global gene expression, chromatin accessibility, histone modification, and transcription factor binding data from purified embryonic stem cell-derived cells representing six sequential stages of hematopoietic specification and differentiation. Our data reveal the nature of regulatory elements driving differential gene expression and inform how transcription factor binding impacts on promoter activity. We present a dynamic core regulatory network model for hematopoietic specification and demonstrate its utility for the design of reprogramming experiments. Functional studies motivated by our genome-wide data uncovered a stage-specific role for TEAD/YAP factors in mammalian hematopoietic specification. Our study presents a powerful resource for studying hematopoiesis and demonstrates how such data advance our understanding of mammalian development.",
author = "Debbie Goode and Constanze Bonifer and Nadine Obier and Jane Gilmour and Salam Assi and Pierre Cauchy and Maarten Hoogenkamp",
year = "2016",
month = mar
day = "7",
doi = "10.1016/j.devcel.2016.01.024",
language = "English",
volume = "36",
pages = "572–587",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation

AU - Goode, Debbie

AU - Bonifer, Constanze

AU - Obier, Nadine

AU - Gilmour, Jane

AU - Assi, Salam

AU - Cauchy, Pierre

AU - Hoogenkamp, Maarten

PY - 2016/3/7

Y1 - 2016/3/7

N2 - Metazoan development involves the successive activation and silencing of specific gene expression programs and is driven by tissue-specific transcription factors programming the chromatin landscape. To understand how this process executes an entire developmental pathway, we generated global gene expression, chromatin accessibility, histone modification, and transcription factor binding data from purified embryonic stem cell-derived cells representing six sequential stages of hematopoietic specification and differentiation. Our data reveal the nature of regulatory elements driving differential gene expression and inform how transcription factor binding impacts on promoter activity. We present a dynamic core regulatory network model for hematopoietic specification and demonstrate its utility for the design of reprogramming experiments. Functional studies motivated by our genome-wide data uncovered a stage-specific role for TEAD/YAP factors in mammalian hematopoietic specification. Our study presents a powerful resource for studying hematopoiesis and demonstrates how such data advance our understanding of mammalian development.

AB - Metazoan development involves the successive activation and silencing of specific gene expression programs and is driven by tissue-specific transcription factors programming the chromatin landscape. To understand how this process executes an entire developmental pathway, we generated global gene expression, chromatin accessibility, histone modification, and transcription factor binding data from purified embryonic stem cell-derived cells representing six sequential stages of hematopoietic specification and differentiation. Our data reveal the nature of regulatory elements driving differential gene expression and inform how transcription factor binding impacts on promoter activity. We present a dynamic core regulatory network model for hematopoietic specification and demonstrate its utility for the design of reprogramming experiments. Functional studies motivated by our genome-wide data uncovered a stage-specific role for TEAD/YAP factors in mammalian hematopoietic specification. Our study presents a powerful resource for studying hematopoiesis and demonstrates how such data advance our understanding of mammalian development.

U2 - 10.1016/j.devcel.2016.01.024

DO - 10.1016/j.devcel.2016.01.024

M3 - Article

C2 - 26923725

VL - 36

SP - 572

EP - 587

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -