Dynamic changes in intrathymic ILC populations during murine neonatal development

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Dynamic changes in intrathymic ILC populations during murine neonatal development. / Jones, Rhys; Cosway, Emilie; Willis, Claire; White, Andrea; Jenkinson, William; Fehling, Hans J.; Anderson, Graham; Withers, David.

In: European Journal of Immunology, Vol. 48, No. 9, 03.09.2018, p. 1481-1491.

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@article{e616abdd949a4a11baf2b444820f1b3a,
title = "Dynamic changes in intrathymic ILC populations during murine neonatal development",
abstract = "Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterisation of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilised multiple in vivo models including the fate mapping of Id2 expression and the use of Id2 reporter mice. Our data demonstrate that ILCs are more prominent immediately after birth, but were rapidly diluted as the T cell development programme increased. As observed in the embryonic thymus, CCR6+ NKp46- lymphoid tissue inducer (LTi) cells were the main ILC3 population present, but numbers of these cells swiftly declined in the neonate and ILC3 were barely detectable in adult thymus. This loss of ILC3 means ILC2 are the dominant ILC population in the thymus. Thymic ILC2 were able to produce IL-5 and IL-13, were located within the medulla and did not result from ILC3 plasticity. Furthermore, in WT mice, thymic ILC2 express little RANKL arguing that functionally, these cells provide different signals to LTi cells in the thymus. Collectively, these data reveal a dynamic switch in the ILC populations of the thymus during neonatal development. This article is protected by copyright. All rights reserved.",
keywords = "innate lymphoid cells, lymphoid tissue, neonate, RORyt, thymus",
author = "Rhys Jones and Emilie Cosway and Claire Willis and Andrea White and William Jenkinson and Fehling, {Hans J.} and Graham Anderson and David Withers",
note = "This article is protected by copyright. All rights reserved.",
year = "2018",
month = sep
day = "3",
doi = "10.1002/eji.201847511",
language = "English",
volume = "48",
pages = "1481--1491",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "9",

}

RIS

TY - JOUR

T1 - Dynamic changes in intrathymic ILC populations during murine neonatal development

AU - Jones, Rhys

AU - Cosway, Emilie

AU - Willis, Claire

AU - White, Andrea

AU - Jenkinson, William

AU - Fehling, Hans J.

AU - Anderson, Graham

AU - Withers, David

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/9/3

Y1 - 2018/9/3

N2 - Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterisation of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilised multiple in vivo models including the fate mapping of Id2 expression and the use of Id2 reporter mice. Our data demonstrate that ILCs are more prominent immediately after birth, but were rapidly diluted as the T cell development programme increased. As observed in the embryonic thymus, CCR6+ NKp46- lymphoid tissue inducer (LTi) cells were the main ILC3 population present, but numbers of these cells swiftly declined in the neonate and ILC3 were barely detectable in adult thymus. This loss of ILC3 means ILC2 are the dominant ILC population in the thymus. Thymic ILC2 were able to produce IL-5 and IL-13, were located within the medulla and did not result from ILC3 plasticity. Furthermore, in WT mice, thymic ILC2 express little RANKL arguing that functionally, these cells provide different signals to LTi cells in the thymus. Collectively, these data reveal a dynamic switch in the ILC populations of the thymus during neonatal development. This article is protected by copyright. All rights reserved.

AB - Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterisation of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilised multiple in vivo models including the fate mapping of Id2 expression and the use of Id2 reporter mice. Our data demonstrate that ILCs are more prominent immediately after birth, but were rapidly diluted as the T cell development programme increased. As observed in the embryonic thymus, CCR6+ NKp46- lymphoid tissue inducer (LTi) cells were the main ILC3 population present, but numbers of these cells swiftly declined in the neonate and ILC3 were barely detectable in adult thymus. This loss of ILC3 means ILC2 are the dominant ILC population in the thymus. Thymic ILC2 were able to produce IL-5 and IL-13, were located within the medulla and did not result from ILC3 plasticity. Furthermore, in WT mice, thymic ILC2 express little RANKL arguing that functionally, these cells provide different signals to LTi cells in the thymus. Collectively, these data reveal a dynamic switch in the ILC populations of the thymus during neonatal development. This article is protected by copyright. All rights reserved.

KW - innate lymphoid cells

KW - lymphoid tissue

KW - neonate

KW - RORyt

KW - thymus

U2 - 10.1002/eji.201847511

DO - 10.1002/eji.201847511

M3 - Article

C2 - 29851080

VL - 48

SP - 1481

EP - 1491

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 9

ER -