Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination

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Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination. / de la Vega Gallardo, Nira ; Penalva, Rosana ; Dittmer , Marie ; Naughton, Michelle ; Falconer, John ; Moffat, Jill ; de la Fuente , Alerie G.; Romero, Jose; Lin, Zhiyong; Perbal, Bernard ; Ingram, Rebecca J .; Evergren, Emma ; Fitzgerald, Denise C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, No. 30, 10.07.2020, p. 18018-18028.

Research output: Contribution to journalArticlepeer-review

Harvard

de la Vega Gallardo, N, Penalva, R, Dittmer , M, Naughton, M, Falconer, J, Moffat, J, de la Fuente , AG, Romero, J, Lin, Z, Perbal, B, Ingram, RJ, Evergren, E & Fitzgerald, DC 2020, 'Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 30, pp. 18018-18028. https://doi.org/10.1073/pnas.1922089117

APA

de la Vega Gallardo, N., Penalva, R., Dittmer , M., Naughton, M., Falconer, J., Moffat, J., de la Fuente , A. G., Romero, J., Lin, Z., Perbal, B., Ingram, R. J. ., Evergren, E., & Fitzgerald, D. C. (2020). Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination. Proceedings of the National Academy of Sciences of the United States of America, 117(30), 18018-18028. https://doi.org/10.1073/pnas.1922089117

Vancouver

Author

de la Vega Gallardo, Nira ; Penalva, Rosana ; Dittmer , Marie ; Naughton, Michelle ; Falconer, John ; Moffat, Jill ; de la Fuente , Alerie G. ; Romero, Jose ; Lin, Zhiyong ; Perbal, Bernard ; Ingram, Rebecca J . ; Evergren, Emma ; Fitzgerald, Denise C. / Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination. In: Proceedings of the National Academy of Sciences of the United States of America. 2020 ; Vol. 117, No. 30. pp. 18018-18028.

Bibtex

@article{3a772f2c28cc42b682d319c95a5b5cc6,
title = "Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination",
abstract = "CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 during these processes. We found CCN3 to be expressed predominantly by neurons in distinct areas of the CNS, primarily the cerebral cortex, hippocampus, amygdala, suprachiasmatic nuclei, anterior olfactory nuclei, and spinal cord gray matter. CCN3 was transiently up-regulated following demyelination in the brain of cuprizone-fed mice and spinal cord lesions of mice injected with lysolecithin. However, CCN3-/- mice did not exhibit significantly different numbers of oligodendroglia or differentiated oligodendrocytes in the healthy or remyelinating CNS, compared to WT controls. These results suggest that despite robust and dynamic expression in the CNS, CCN3 is not required for efficient myelination or remyelination in the murine CNS in vivo. ",
keywords = "CCN3, Myelin, OPC, Oligodendrocyte, Remyelination, Gene Expression Regulation, Spinal Cord/metabolism, Oligodendrocyte Precursor Cells/metabolism, Central Nervous System/metabolism, Animals, Remyelination/genetics, Fluorescent Antibody Technique, Oligodendroglia/metabolism, Nephroblastoma Overexpressed Protein/genetics, Mice, Myelin Sheath/metabolism, Demyelinating Diseases/etiology, Brain/metabolism, Disease Models, Animal",
author = "{de la Vega Gallardo}, Nira and Rosana Penalva and Marie Dittmer and Michelle Naughton and John Falconer and Jill Moffat and {de la Fuente}, {Alerie G.} and Jose Romero and Zhiyong Lin and Bernard Perbal and Ingram, {Rebecca J .} and Emma Evergren and Fitzgerald, {Denise C.}",
note = "Funding Information: ACKNOWLEDGMENTS. We thank Prof. Anna Williams and her team for kindly providing us with the ImageJ macro used to analyse brain slice images in this study. We thank Anna Magennis for technical support and Rachel Jayne Gillis and Sarah Collis for cell quantification. We also thank QUB Biological Services Unit and the advanced imaging unit staff for their help and advice, particularly Andrena Millar and Dr. Ileana Micu. We thank Dr. Yvonne Dombrowski{\textquoteright}s laboratory, Dr. Liza Colhoun, and WWIEM immunology laboratory groups for their advice and guidance through the course of this study. This work was supported by Biotechnology and Biological Sciences Research Council Grant BB/J01026X/1 (to D.C.F.), Wellcome Trust Grant 110138/Z/15/Z (to D.C.F.), and studentship support from the Department for the Economy (Northern Ireland). Publisher Copyright: {\textcopyright} 2020 National Academy of Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jul,
day = "10",
doi = "10.1073/pnas.1922089117",
language = "English",
volume = "117",
pages = "18018--18028",
journal = "Proceedings of the National Academy of Sciences",
issn = "1091-6490",
publisher = "National Academy of Sciences",
number = "30",

}

RIS

TY - JOUR

T1 - Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination

AU - de la Vega Gallardo, Nira

AU - Penalva, Rosana

AU - Dittmer , Marie

AU - Naughton, Michelle

AU - Falconer, John

AU - Moffat, Jill

AU - de la Fuente , Alerie G.

AU - Romero, Jose

AU - Lin, Zhiyong

AU - Perbal, Bernard

AU - Ingram, Rebecca J .

AU - Evergren, Emma

AU - Fitzgerald, Denise C.

N1 - Funding Information: ACKNOWLEDGMENTS. We thank Prof. Anna Williams and her team for kindly providing us with the ImageJ macro used to analyse brain slice images in this study. We thank Anna Magennis for technical support and Rachel Jayne Gillis and Sarah Collis for cell quantification. We also thank QUB Biological Services Unit and the advanced imaging unit staff for their help and advice, particularly Andrena Millar and Dr. Ileana Micu. We thank Dr. Yvonne Dombrowski’s laboratory, Dr. Liza Colhoun, and WWIEM immunology laboratory groups for their advice and guidance through the course of this study. This work was supported by Biotechnology and Biological Sciences Research Council Grant BB/J01026X/1 (to D.C.F.), Wellcome Trust Grant 110138/Z/15/Z (to D.C.F.), and studentship support from the Department for the Economy (Northern Ireland). Publisher Copyright: © 2020 National Academy of Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/10

Y1 - 2020/7/10

N2 - CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 during these processes. We found CCN3 to be expressed predominantly by neurons in distinct areas of the CNS, primarily the cerebral cortex, hippocampus, amygdala, suprachiasmatic nuclei, anterior olfactory nuclei, and spinal cord gray matter. CCN3 was transiently up-regulated following demyelination in the brain of cuprizone-fed mice and spinal cord lesions of mice injected with lysolecithin. However, CCN3-/- mice did not exhibit significantly different numbers of oligodendroglia or differentiated oligodendrocytes in the healthy or remyelinating CNS, compared to WT controls. These results suggest that despite robust and dynamic expression in the CNS, CCN3 is not required for efficient myelination or remyelination in the murine CNS in vivo.

AB - CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 during these processes. We found CCN3 to be expressed predominantly by neurons in distinct areas of the CNS, primarily the cerebral cortex, hippocampus, amygdala, suprachiasmatic nuclei, anterior olfactory nuclei, and spinal cord gray matter. CCN3 was transiently up-regulated following demyelination in the brain of cuprizone-fed mice and spinal cord lesions of mice injected with lysolecithin. However, CCN3-/- mice did not exhibit significantly different numbers of oligodendroglia or differentiated oligodendrocytes in the healthy or remyelinating CNS, compared to WT controls. These results suggest that despite robust and dynamic expression in the CNS, CCN3 is not required for efficient myelination or remyelination in the murine CNS in vivo.

KW - CCN3

KW - Myelin

KW - OPC

KW - Oligodendrocyte

KW - Remyelination

KW - Gene Expression Regulation

KW - Spinal Cord/metabolism

KW - Oligodendrocyte Precursor Cells/metabolism

KW - Central Nervous System/metabolism

KW - Animals

KW - Remyelination/genetics

KW - Fluorescent Antibody Technique

KW - Oligodendroglia/metabolism

KW - Nephroblastoma Overexpressed Protein/genetics

KW - Mice

KW - Myelin Sheath/metabolism

KW - Demyelinating Diseases/etiology

KW - Brain/metabolism

KW - Disease Models, Animal

UR - http://www.scopus.com/inward/record.url?scp=85088879787&partnerID=8YFLogxK

U2 - 10.1073/pnas.1922089117

DO - 10.1073/pnas.1922089117

M3 - Article

C2 - 32651278

VL - 117

SP - 18018

EP - 18028

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 30

ER -