Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard; Adam C. Sedgwick; Thitima Sombuttan; George T. Williams; Boontana Wannalerse; A. Toby A. Jenkins; Steven D. Bull; Tony D. James

doi:10.1002/open.201700193

Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard, Adam C. Sedgwick, Thitima Sombuttan, George T. Williams, Boontana Wannalerse, A. Toby A. Jenkins, Steven D. Bull, Tony D. James

Chemistry

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

Original language	English
Pages (from-to)	266-268
Journal	ChemistryOpen
Volume	7
Issue number	3
DOIs	https://doi.org/10.1002/open.201700193
Publication status	Published - Mar 2018

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title = "Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels",

abstract = "Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.",

author = "Lampard, {Emma V.} and Sedgwick, {Adam C.} and Thitima Sombuttan and Williams, {George T.} and Boontana Wannalerse and Jenkins, {A. Toby A.} and Bull, {Steven D.} and James, {Tony D.}",

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T1 - Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

AU - Lampard, Emma V.

AU - Sedgwick, Adam C.

AU - Sombuttan, Thitima

AU - Williams, George T.

AU - Wannalerse, Boontana

AU - Jenkins, A. Toby A.

AU - Bull, Steven D.

AU - James, Tony D.

PY - 2018/3

Y1 - 2018/3

N2 - Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

AB - Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

UR - https://doi.org/10.1002/open.201700193

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DO - 10.1002/open.201700193

M3 - Article

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EP - 268

JO - ChemistryOpen

JF - ChemistryOpen

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ER -

Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Abstract

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