Dried blood spot proteomics : surface extraction of endogenous proteins coupled with automated sample preparation and mass spectrometry analysis

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

Abstract

Dried blood spots offer many advantages as a sample format including ease and safety of transport and handling. To date, the majority of mass spectrometry analyses of dried blood spots have focused on small molecules or hemoglobin. However, dried blood spots are a potentially rich source of protein biomarkers, an area that has been overlooked. To address this issue, we have applied an untargeted bottom-up proteomics approach to the analysis of dried blood spots. We present an automated and integrated method for extraction of endogenous proteins from the surface of dried blood spots and sample preparation via trypsin digestion by use of the Advion Biosciences Triversa Nanomate robotic platform. Liquid chromatography tandem mass spectrometry of the resulting digests enabled identification of 120 proteins from a single dried blood spot. The proteins identified cross a concentration range of four orders of magnitude. The method is evaluated and the results discussed in terms of the proteins identified and their potential use as biomarkers in screening programs.

Details

Original languageEnglish
Pages (from-to)1242-1249
JournalJournal of the American Society for Mass Spectrometry
Volume24
Issue number8
Publication statusPublished - 1 Aug 2013

Keywords

  • Dried blood spots, Proteomics, Direct surface sampling, Liquid extraction surface analysis, Automated sample preparation