Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma

Domokos Bartis; Veronika Csongei; Alexander Weich; Edit Kiss; Szilvia Barko; Tamas Kovacs; Monika Avdicevic; Vijay K. D'Souza; Judit Rapp; Krisztian Kvell; Laszlo Jakab; Miklos Nyitrai; Tamas F. Molnar; David R. Thickett; Terezia Laszlo; Judit E. Pongracz

doi:10.1371/journal.pone.0057393

Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma

Domokos Bartis, Veronika Csongei, Alexander Weich, Edit Kiss, Szilvia Barko, Tamas Kovacs, Monika Avdicevic, Vijay K. D'Souza, Judit Rapp, Krisztian Kvell, Laszlo Jakab, Miklos Nyitrai, Tamas F. Molnar, David R. Thickett, Terezia Laszlo, Judit E. Pongracz

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The majority of lung cancers (LC) belong to the non-small cell lung carcinoma (NSCLC) type. The two main NSCLC sub-types, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC), respond differently to therapy. Whereas the link between cigarette smoke and lung cancer risk is well established, the relevance of non-canonical Wnt pathway up-regulation detected in SCC remains poorly understood. The present study was undertaken to investigate further the molecular events in canonical and non-canonical Wnt signalling during SCC development. A total of 20 SCC and AC samples with matched non-cancerous controls were obtained after surgery. TaqMan array analysis confirmed up-regulation of non-canonical Wnt5a and Wnt11 and identified down-regulation of canonical Wnt signalling in SCC samples. The molecular changes were tested in primary small airway epithelial cells (SAEC) and various lung cancer cell lines (e.g. A549, H157, etc). Our studies identified Wnt11 and Wnt5a as regulators of cadherin expression and potentiated relocation of β-catenin to the nucleus as an important step in decreased cellular adhesion. The presented data identifies additional details in the regulation of SCC that can aid identification of therapeutic drug targets in the future.

Original language	English
Article number	e57393
Journal	PLoS ONE
Volume	8
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0057393
Publication status	Published - 7 Mar 2013

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pone.0057393

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Bartis, D., Csongei, V., Weich, A., Kiss, E., Barko, S., Kovacs, T., Avdicevic, M., D'Souza, V. K., Rapp, J., Kvell, K., Jakab, L., Nyitrai, M., Molnar, T. F., Thickett, D. R., Laszlo, T., & Pongracz, J. E. (2013). Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma. PLoS ONE, 8(3), Article e57393. https://doi.org/10.1371/journal.pone.0057393

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title = "Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma",

abstract = "The majority of lung cancers (LC) belong to the non-small cell lung carcinoma (NSCLC) type. The two main NSCLC sub-types, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC), respond differently to therapy. Whereas the link between cigarette smoke and lung cancer risk is well established, the relevance of non-canonical Wnt pathway up-regulation detected in SCC remains poorly understood. The present study was undertaken to investigate further the molecular events in canonical and non-canonical Wnt signalling during SCC development. A total of 20 SCC and AC samples with matched non-cancerous controls were obtained after surgery. TaqMan array analysis confirmed up-regulation of non-canonical Wnt5a and Wnt11 and identified down-regulation of canonical Wnt signalling in SCC samples. The molecular changes were tested in primary small airway epithelial cells (SAEC) and various lung cancer cell lines (e.g. A549, H157, etc). Our studies identified Wnt11 and Wnt5a as regulators of cadherin expression and potentiated relocation of β-catenin to the nucleus as an important step in decreased cellular adhesion. The presented data identifies additional details in the regulation of SCC that can aid identification of therapeutic drug targets in the future.",

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Bartis, D, Csongei, V, Weich, A, Kiss, E, Barko, S, Kovacs, T, Avdicevic, M, D'Souza, VK, Rapp, J, Kvell, K, Jakab, L, Nyitrai, M, Molnar, TF, Thickett, DR, Laszlo, T & Pongracz, JE 2013, 'Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma', PLoS ONE, vol. 8, no. 3, e57393. https://doi.org/10.1371/journal.pone.0057393

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AU - Bartis, Domokos

AU - Csongei, Veronika

AU - Weich, Alexander

AU - Kiss, Edit

AU - Barko, Szilvia

AU - Kovacs, Tamas

AU - Avdicevic, Monika

AU - D'Souza, Vijay K.

AU - Rapp, Judit

AU - Kvell, Krisztian

AU - Jakab, Laszlo

AU - Nyitrai, Miklos

AU - Molnar, Tamas F.

AU - Thickett, David R.

AU - Laszlo, Terezia

AU - Pongracz, Judit E.

PY - 2013/3/7

Y1 - 2013/3/7

N2 - The majority of lung cancers (LC) belong to the non-small cell lung carcinoma (NSCLC) type. The two main NSCLC sub-types, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC), respond differently to therapy. Whereas the link between cigarette smoke and lung cancer risk is well established, the relevance of non-canonical Wnt pathway up-regulation detected in SCC remains poorly understood. The present study was undertaken to investigate further the molecular events in canonical and non-canonical Wnt signalling during SCC development. A total of 20 SCC and AC samples with matched non-cancerous controls were obtained after surgery. TaqMan array analysis confirmed up-regulation of non-canonical Wnt5a and Wnt11 and identified down-regulation of canonical Wnt signalling in SCC samples. The molecular changes were tested in primary small airway epithelial cells (SAEC) and various lung cancer cell lines (e.g. A549, H157, etc). Our studies identified Wnt11 and Wnt5a as regulators of cadherin expression and potentiated relocation of β-catenin to the nucleus as an important step in decreased cellular adhesion. The presented data identifies additional details in the regulation of SCC that can aid identification of therapeutic drug targets in the future.

AB - The majority of lung cancers (LC) belong to the non-small cell lung carcinoma (NSCLC) type. The two main NSCLC sub-types, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC), respond differently to therapy. Whereas the link between cigarette smoke and lung cancer risk is well established, the relevance of non-canonical Wnt pathway up-regulation detected in SCC remains poorly understood. The present study was undertaken to investigate further the molecular events in canonical and non-canonical Wnt signalling during SCC development. A total of 20 SCC and AC samples with matched non-cancerous controls were obtained after surgery. TaqMan array analysis confirmed up-regulation of non-canonical Wnt5a and Wnt11 and identified down-regulation of canonical Wnt signalling in SCC samples. The molecular changes were tested in primary small airway epithelial cells (SAEC) and various lung cancer cell lines (e.g. A549, H157, etc). Our studies identified Wnt11 and Wnt5a as regulators of cadherin expression and potentiated relocation of β-catenin to the nucleus as an important step in decreased cellular adhesion. The presented data identifies additional details in the regulation of SCC that can aid identification of therapeutic drug targets in the future.

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Down-Regulation of Canonical and Up-Regulation of Non-Canonical Wnt Signalling in the Carcinogenic Process of Squamous Cell Lung Carcinoma

Abstract

ASJC Scopus subject areas

UN SDGs

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