DNA sequence properties that predict susceptibility to epiallelic switching

Research output: Contribution to journalArticlepeer-review

Authors

  • Jayne Griffiths
  • Claude Becker
  • Nicolae Radu Zabet
  • Carlos Bayon
  • Mélanie Dapp
  • Michal Lieberman-Lazarovich
  • Detlef Weigel
  • Jerzy Paszkowski

Colleges, School and Institutes

External organisations

  • Department of Plant Biology, University of Geneva, Geneva, Switzerland.
  • Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
  • University of Cambridge

Abstract

Transgenerationally heritable epialleles are defined by the stable propagation of alternative transcriptional states through mitotic and meiotic cell cycles. Given that the propagation of DNA methylation at CpG sites, mediated in Arabidopsis by MET1, plays a central role in epigenetic inheritance, we examined genomewide DNA methylation in partial and complete loss-of-function met1 mutants. We interpreted the data in relation to transgenerational epiallelic stability, which allowed us to classify chromosomal targets of epigenetic regulation into (i) single copy and methylated exclusively at CpGs, readily forming epialleles, and (ii) transposon-derived, methylated at all cytosines, which may or may not form epialleles. We provide evidence that DNA sequence features such as density of CpGs and genomic repetitiveness of the loci predispose their susceptibility to epiallelic switching. The importance and predictive power of these genetic features were confirmed by analyses of common epialleles in natural Arabidopsis accessions, epigenetic recombinant inbred lines (epiRILs) and also verified in rice.

Details

Original languageEnglish
Pages (from-to)617-628
Number of pages12
JournalThe EMBO journal
Volume36
Issue number5
Early online date9 Jan 2017
Publication statusPublished - 1 Mar 2017

Keywords

  • Arabidopsis/genetics, Arabidopsis Proteins/genetics, DNA (Cytosine-5-)-Methyltransferases/genetics, DNA Methylation, DNA, Plant/chemistry, Epigenesis, Genetic, Gene Expression Regulation, Plant, Mutation