DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes.
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter.
|Journal||Nucleic Acids Research|
|Publication status||Published - 30 Jan 2009|