DNA barcodes for rapid, whole genome, single-molecule analyses

Research output: Contribution to journalArticle

Standard

DNA barcodes for rapid, whole genome, single-molecule analyses. / Wand, Nathaniel; Smith, Darren A; Wilkinson, Andrew; Rushton, Ashleigh; Busby, Stephen J. W.; Styles, Iain; Neely, Robert K.

In: Nucleic Acids Research, Vol. 47, No. 12, gkz212, 09.07.2019, p. e68.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Bibtex

@article{c2f965bc4194422b8dea44fa2a285d4a,
title = "DNA barcodes for rapid, whole genome, single-molecule analyses",
abstract = "We report an approach for visualizing DNA sequence and using these {\textquoteleft}DNA barcodes{\textquoteright} to search complex mixtures of genomic material for DNA molecules of interest. We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.",
keywords = "DNA barcodes, Methyltransferases",
author = "Nathaniel Wand and Smith, {Darren A} and Andrew Wilkinson and Ashleigh Rushton and Busby, {Stephen J. W.} and Iain Styles and Neely, {Robert K.}",
note = "{\textcopyright} The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2019",
month = jul,
day = "9",
doi = "10.1093/nar/gkz212",
language = "English",
volume = "47",
pages = "e68",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "12",

}

RIS

TY - JOUR

T1 - DNA barcodes for rapid, whole genome, single-molecule analyses

AU - Wand, Nathaniel

AU - Smith, Darren A

AU - Wilkinson, Andrew

AU - Rushton, Ashleigh

AU - Busby, Stephen J. W.

AU - Styles, Iain

AU - Neely, Robert K.

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2019/7/9

Y1 - 2019/7/9

N2 - We report an approach for visualizing DNA sequence and using these ‘DNA barcodes’ to search complex mixtures of genomic material for DNA molecules of interest. We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.

AB - We report an approach for visualizing DNA sequence and using these ‘DNA barcodes’ to search complex mixtures of genomic material for DNA molecules of interest. We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.

KW - DNA barcodes

KW - Methyltransferases

UR - https://doi.org/10.1101/450809

U2 - 10.1093/nar/gkz212

DO - 10.1093/nar/gkz212

M3 - Article

C2 - 30918971

VL - 47

SP - e68

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 12

M1 - gkz212

ER -