DNA barcodes for rapid, whole genome, single-molecule analyses

Research output: Contribution to journalArticle

Authors

  • Nathaniel Wand
  • Andrew Wilkinson
  • Ashleigh Rushton

Colleges, School and Institutes

Abstract

We report an approach for visualizing DNA sequence and using these ‘DNA barcodes’ to search complex mixtures of genomic material for DNA molecules of interest. We demonstrate three applications of this methodology; identifying specific molecules of interest from a dataset containing gigabasepairs of genome; identification of a bacterium from such a dataset and, finally, by locating infecting virus molecules in a background of human genomic material. As a result of the dense fluorescent labelling of the DNA, individual barcodes of the order 40 kb pairs in length can be reliably identified. This means DNA can be prepared for imaging using standard handling and purification techniques. The recorded dataset provides stable physical and electronic records of the total genomic content of a sample that can be readily searched for a molecule or region of interest.

Details

Original languageEnglish
Article numbergkz212
Number of pages10
JournalNucleic Acids Research
Early online date28 Mar 2019
Publication statusE-pub ahead of print - 28 Mar 2019

Keywords

  • DNA barcodes, Methyltransferases