Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide

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Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide. / Smith Callahan, L.A.; Xie, S.; Barker, I.A.; Zheng, J.; Reneker, D.H.; Dove, A.P.; Becker, M.L.

In: Biomaterials, Vol. 34, No. 36, 03.09.2013, p. 9089-9095.

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Smith Callahan, L.A. ; Xie, S. ; Barker, I.A. ; Zheng, J. ; Reneker, D.H. ; Dove, A.P. ; Becker, M.L. / Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide. In: Biomaterials. 2013 ; Vol. 34, No. 36. pp. 9089-9095.

Bibtex

@article{bd7c668021de42b88ab9fe2e12bdf6b0,
title = "Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide",
abstract = "End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free “click chemistry” with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.",
keywords = "PLLA, functionalization, degradable, polymers",
author = "{Smith Callahan}, L.A. and S. Xie and I.A. Barker and J. Zheng and D.H. Reneker and A.P. Dove and M.L. Becker",
year = "2013",
month = sep,
day = "3",
doi = "10.1016/j.biomaterials.2013.08.028",
language = "Undefined/Unknown",
volume = "34",
pages = "9089--9095",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Doyma",
number = "36",

}

RIS

TY - JOUR

T1 - Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide

AU - Smith Callahan, L.A.

AU - Xie, S.

AU - Barker, I.A.

AU - Zheng, J.

AU - Reneker, D.H.

AU - Dove, A.P.

AU - Becker, M.L.

PY - 2013/9/3

Y1 - 2013/9/3

N2 - End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free “click chemistry” with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.

AB - End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free “click chemistry” with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.

KW - PLLA

KW - functionalization

KW - degradable

KW - polymers

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84883820106&partnerID=MN8TOARS

U2 - 10.1016/j.biomaterials.2013.08.028

DO - 10.1016/j.biomaterials.2013.08.028

M3 - Article

VL - 34

SP - 9089

EP - 9095

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 36

ER -