Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide
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Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide. / Smith Callahan, L.A.; Xie, S.; Barker, I.A.; Zheng, J.; Reneker, D.H.; Dove, A.P.; Becker, M.L.
In: Biomaterials, Vol. 34, No. 36, 03.09.2013, p. 9089-9095.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide
AU - Smith Callahan, L.A.
AU - Xie, S.
AU - Barker, I.A.
AU - Zheng, J.
AU - Reneker, D.H.
AU - Dove, A.P.
AU - Becker, M.L.
PY - 2013/9/3
Y1 - 2013/9/3
N2 - End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free “click chemistry” with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.
AB - End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free “click chemistry” with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.
KW - PLLA
KW - functionalization
KW - degradable
KW - polymers
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84883820106&partnerID=MN8TOARS
U2 - 10.1016/j.biomaterials.2013.08.028
DO - 10.1016/j.biomaterials.2013.08.028
M3 - Article
VL - 34
SP - 9089
EP - 9095
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 36
ER -