Direct interaction between Utp8p and Utp9p contributes to rRNA processing in budding yeast.

Research output: Contribution to journalArticle

Authors

  • Hung-Ji Tsai
  • Shun-Fu Tseng
  • Yu-Ching Huang
  • Brian A. Lenzmeier
  • Shu-Chun Teng

Colleges, School and Institutes

External organisations

  • Department of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan, ROC
  • School of Science, Buena Vista University, 610 West 4th St., Storm Lake, IA 50588, USA

Abstract

The small subunit (SSU) processome is an evolutionarily conserved ribonucleoprotein (RNP) complex that consists of U3 snoRNA and at least 40 protein components. The SSU processome is required for the generation of 18S rRNA in the budding yeast Saccharomyces cerevisiae. In this study we demonstrate that two essential components of the SSU processome, Utp8p and Utp9p, must interact directly for the SSU processome to function properly. Disruption of the Utp8p-Utp9p interaction by mutation of the respective interacting domain led to a compromised ability of yeast cells to process 35S pre-rRNA into 18S pre-rRNA. Loss of the Utp8p-Utp9p interaction also led to a decrease in the amount of Utp8p that interacted with U3 small nucleolar RNAs (snoRNAs) but did not affect the amount of Utp9p bound to U3 snoRNA, suggesting that Utp8p associates with the SSU processome by virtue of its interaction with Utp9p. Together, our data support a model where Utp8p and Utp9p must interact directly and functionally in the U3-containing SSU processome for optimal rRNA biosynthesis to occur in budding yeast.

Details

Original languageEnglish
Pages (from-to)297-302
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number2
Publication statusPublished - 6 Feb 2010