Diol Appended Quenchers for Fluorescein Boronic Acid

Research output: Contribution to journalArticle

Authors

  • Souad Elfeky
  • SE Flower
  • N Masumoto
  • F D'Hooge
  • L Labarthe
  • C Len
  • TD James

Colleges, School and Institutes

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

Details

Original languageEnglish
Pages (from-to)581-588
Number of pages8
JournalChemistry - An Asian Journal
Volume5
Issue number3
Publication statusPublished - 1 Jan 2010

Keywords

  • nucleoside, receptor, fluorophore, boronic acid, quencher