Projects per year
Abstract
BACKGROUND: Sixty percent of people with coeliac disease (CD) are iron deficient. Many, however, remain iron replete despite the disease. AIMS: (i) To characterize the changes in duodenal iron transport proteins in CD with and without iron deficiency. (ii) To examine if iron-activated gut lymphocytes can inhibit iron export in an enterocyte cell model. METHODS: Endoscopic duodenal biopsies were collected from patients with normal duodenum with and without iron deficiency anaemia and untreated CD sufferers with iron deficiency (n=10 each group). mRNA expression of iron transport proteins was determined by quantitative real time PCR. Protein localization and expression was determined from histology sections in patients with normal duodenum (n=20), and patients with untreated CD with and without iron deficiency (n=20). In addition, CaCo2 cells were cocultured with iron-activated lymphocytes 55Fe was used to determine the effect on CaCo2 cell iron transport. RESULTS: The expression of divalent metal transporter 1 and ferroportin was increased in CD with or without iron deficiency. Ferritin expression was increased in CD but only in those with associated iron deficiency. TNF-alpha produced by activated lymphocytes inhibited iron export from CaCo2 cells. CONCLUSION: Increased enterocyte ferritin expression may promote iron deficiency in CD and this effect seems to be dependent upon TNF-alpha expression in gut lymphocytes.
Original language | English |
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Pages (from-to) | 794-804 |
Number of pages | 11 |
Journal | European Journal of Gastroenterology & Hepatology |
Volume | 21 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Jul 2009 |
Keywords
- tumour necrosis factor-alpha
- divalent metal transporter 1
- ferritin
- iron
- coeliac disease
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Dive into the research topics of 'Differential ferritin expression is associated with iron deficiency in coeliac disease.'. Together they form a unique fingerprint.Projects
- 2 Finished
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Mucosal Lymphocytes in the Pathogenesis of Primary Sclerosing Cholangitis
Eksteen, B.
1/10/07 → 31/03/13
Project: Research Councils
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Characterisation of Iron Transport Proteins and their Modulation by TNF-a and Hepcidin in Coeliac Disease
Biotechnology & Biological Sciences Research Council
1/04/04 → 31/08/07
Project: Research Councils