Differential expression of inflammasome regulatory transcripts in periodontal disease

Research output: Contribution to journalArticle

Authors

  • Kübra Aral
  • Eynar Berdeli
  • Yvonne Kapila
  • Beyza Karadede Ünal
  • Cüneyt Asım Aral
  • Afig Berdeli

Colleges, School and Institutes

External organisations

  • Oral Biology, School of DentistryUniversity of Birmingham Birmingham UK
  • Faculty of DentistryIzmir Katip Celebi University Izmir Turkey
  • The School of DentistryUniversity of California San Francisco San Francisco CA USA
  • Faculty of DentistryInonu University Malatya Turkey
  • Faculty of Medicine, Molecular Medicine LaboratoryEge University Izmir Turkey

Abstract

Background:The inflammasome modulates the release of key proinflammatory cytokines associated with periodontal disease pathogenesis. The aim of this study was to evaluate the expression of proteins that regulate the inflammasome, namely pyrin domain‐only proteins (POPs), caspase activation recruitment domain (CARD)‐only proteins, and tripartite motif‐containing (TRIM) proteins, in periodontal diseases.
Methods:A total of 68 participants (34 males and 34 females) were divided into four groups, including periodontal health (H), gingivitis (G), chronic periodontitis (CP), and aggressive periodontitis (AgP) based on clinical parameters. Gingival tissue samples were obtained from all participants for reverse transcription polymerase chain reaction (RT‐PCR)‐based gene expression analyses of molecules that regulate the inflammasome, including apoptosis‐associated speck‐like protein (ASC) containing CARD, caspase‐1, interleukin‐1β (IL‐1β), interleukin‐18 (IL‐18), nucleotide‐binding domain, leucine rich family (NLR) pyrin domain containing 3 (NLRP3), NLR family pyrin domain containing 2 (NLRP2), AIM2 (absent in melanoma 2), POP1, POP2, CARD16, CARD18, TRIM16, and TRIM20 by RT‐PCR.
Results:NLRP3 and IL‐1β were upregulated in the G, CP, and AgP groups compared with group H (P < 0.05). AIM2 was downregulated in the CP group compared with the H, G, and AgP groups (P < 0.05). TRIM20, TRIM16, and CARD18 were downregulated in the G, CP, and AgP groups compared with the H group (P < 0.05). POP1 and POP2 were downregulated in the CP and AgP, and AgP and G groups, respectively (P < 0.05).
Conclusion:Active periodontal disease may result in downregulation of inflammasome regulators that may increase the activity of NLRP3 and IL‐1β in periodontal disease.

Details

Original languageEnglish
Number of pages11
JournalJournal of Periodontology
Early online date26 Sep 2019
Publication statusE-pub ahead of print - 26 Sep 2019

Keywords

  • caspase activation and recruitment domains, inflammasomes, periodontal disease, pyrin domain, tripartitemotif proteins