Dietary n-3 PUFA May Attenuate Experimental Colitis

Cloé Charpentier; Ronald Chan; Emmeline Salameh; Khaly Mbodji; Aito Ueno; Moïse Coëffier; Charlène Guérin; Subrata Ghosh; Guillaume Savoye; Rachel Marion-Letellier

doi:10.1155/2018/8430614

Dietary n-3 PUFA May Attenuate Experimental Colitis

Cloé Charpentier, Ronald Chan, Emmeline Salameh, Khaly Mbodji, Aito Ueno, Moïse Coëffier, Charlène Guérin, Subrata Ghosh, Guillaume Savoye, Rachel Marion-Letellier

Immunology and Immunotherapy

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Abstract

Background: Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected to contribute to the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation in rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary fatty acid pattern may influence intestinal environment to modify inflammation genesis. The aim of this study was to evaluate the dietary effect of PUFA composition on intestinal inflammation.

Methods: Animals received diet varying in their PUFA composition for four weeks before TNBS-induced colitis. Colon inflammatory markers and gut barrier function parameters were assessed. Inflammatory pathway PCR arrays were determined.

Results: n-3 diet significantly decreased colon iNOS, COX-2 expression, IL-6 production, and LTB4 production but tended to decrease colon TNFα production (P = 0.0617) compared to control diet. Tight junction protein (claudin-1, occludin) expressions and MUC2 and TFF3 mRNA levels were not different among groups. n-9 diet also decreased colon IL-6 production (P < 0.05).

Conclusions: Dietary n-3 PUFA influence colitis development by attenuating inflammatory markers. Further research is required to better define dietary advice with a scientific rationale.

Original language	English
Article number	8430614
Number of pages	10
Journal	Mediators of Inflammation
Volume	2018
DOIs	https://doi.org/10.1155/2018/8430614 https://doi.org/10.1155/2018/8430614
Publication status	Published - 15 Feb 2018

Keywords

Animals
Claudin-1/metabolism
Colitis/drug therapy
Cyclooxygenase 2/metabolism
Disease Models, Animal
Fatty Acids, Omega-3/therapeutic use
Inflammatory Bowel Diseases/drug therapy
Interleukin-6/metabolism
Leukotriene B4/metabolism
Male
Nitric Oxide Synthase Type II/metabolism
Occludin/metabolism
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha/metabolism

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Charpentier_et_al_Dietary_n-3_PUFA_Mediators_of_Inflammation_2018
Checked for eligibility: 28/09/2018 First published in: Mediators of Inflammation Volume 2018, Article ID 8430614, 10 pages https://doi.org/10.1155/2018/8430614
Final published version, 2.66 MBLicence: Creative Commons: Attribution (CC BY)

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@article{b1234f1040854cc69dd2b2260d057556,

title = "Dietary n-3 PUFA May Attenuate Experimental Colitis",

abstract = "Background: Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected to contribute to the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation in rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary fatty acid pattern may influence intestinal environment to modify inflammation genesis. The aim of this study was to evaluate the dietary effect of PUFA composition on intestinal inflammation.Methods: Animals received diet varying in their PUFA composition for four weeks before TNBS-induced colitis. Colon inflammatory markers and gut barrier function parameters were assessed. Inflammatory pathway PCR arrays were determined.Results: n-3 diet significantly decreased colon iNOS, COX-2 expression, IL-6 production, and LTB4 production but tended to decrease colon TNFα production (P = 0.0617) compared to control diet. Tight junction protein (claudin-1, occludin) expressions and MUC2 and TFF3 mRNA levels were not different among groups. n-9 diet also decreased colon IL-6 production (P < 0.05).Conclusions: Dietary n-3 PUFA influence colitis development by attenuating inflammatory markers. Further research is required to better define dietary advice with a scientific rationale.",

keywords = "Animals, Claudin-1/metabolism, Colitis/drug therapy, Cyclooxygenase 2/metabolism, Disease Models, Animal, Fatty Acids, Omega-3/therapeutic use, Inflammatory Bowel Diseases/drug therapy, Interleukin-6/metabolism, Leukotriene B4/metabolism, Male, Nitric Oxide Synthase Type II/metabolism, Occludin/metabolism, Rats, Rats, Sprague-Dawley, Tumor Necrosis Factor-alpha/metabolism",

author = "Clo{\'e} Charpentier and Ronald Chan and Emmeline Salameh and Khaly Mbodji and Aito Ueno and Mo{\"i}se Co{\"e}ffier and Charl{\`e}ne Gu{\'e}rin and Subrata Ghosh and Guillaume Savoye and Rachel Marion-Letellier",

year = "2018",

month = feb,

day = "15",

doi = "10.1155/2018/8430614",

language = "English",

volume = "2018",

journal = "Mediators of Inflammation",

issn = "0962-9351",

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T1 - Dietary n-3 PUFA May Attenuate Experimental Colitis

AU - Charpentier, Cloé

AU - Chan, Ronald

AU - Salameh, Emmeline

AU - Mbodji, Khaly

AU - Ueno, Aito

AU - Coëffier, Moïse

AU - Guérin, Charlène

AU - Ghosh, Subrata

AU - Savoye, Guillaume

AU - Marion-Letellier, Rachel

PY - 2018/2/15

Y1 - 2018/2/15

N2 - Background: Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected to contribute to the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation in rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary fatty acid pattern may influence intestinal environment to modify inflammation genesis. The aim of this study was to evaluate the dietary effect of PUFA composition on intestinal inflammation.Methods: Animals received diet varying in their PUFA composition for four weeks before TNBS-induced colitis. Colon inflammatory markers and gut barrier function parameters were assessed. Inflammatory pathway PCR arrays were determined.Results: n-3 diet significantly decreased colon iNOS, COX-2 expression, IL-6 production, and LTB4 production but tended to decrease colon TNFα production (P = 0.0617) compared to control diet. Tight junction protein (claudin-1, occludin) expressions and MUC2 and TFF3 mRNA levels were not different among groups. n-9 diet also decreased colon IL-6 production (P < 0.05).Conclusions: Dietary n-3 PUFA influence colitis development by attenuating inflammatory markers. Further research is required to better define dietary advice with a scientific rationale.

AB - Background: Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected to contribute to the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation in rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary fatty acid pattern may influence intestinal environment to modify inflammation genesis. The aim of this study was to evaluate the dietary effect of PUFA composition on intestinal inflammation.Methods: Animals received diet varying in their PUFA composition for four weeks before TNBS-induced colitis. Colon inflammatory markers and gut barrier function parameters were assessed. Inflammatory pathway PCR arrays were determined.Results: n-3 diet significantly decreased colon iNOS, COX-2 expression, IL-6 production, and LTB4 production but tended to decrease colon TNFα production (P = 0.0617) compared to control diet. Tight junction protein (claudin-1, occludin) expressions and MUC2 and TFF3 mRNA levels were not different among groups. n-9 diet also decreased colon IL-6 production (P < 0.05).Conclusions: Dietary n-3 PUFA influence colitis development by attenuating inflammatory markers. Further research is required to better define dietary advice with a scientific rationale.

KW - Animals

KW - Claudin-1/metabolism

KW - Colitis/drug therapy

KW - Cyclooxygenase 2/metabolism

KW - Disease Models, Animal

KW - Fatty Acids, Omega-3/therapeutic use

KW - Inflammatory Bowel Diseases/drug therapy

KW - Interleukin-6/metabolism

KW - Leukotriene B4/metabolism

KW - Male

KW - Nitric Oxide Synthase Type II/metabolism

KW - Occludin/metabolism

KW - Rats

KW - Rats, Sprague-Dawley

KW - Tumor Necrosis Factor-alpha/metabolism

UR - https://doi.org/10.1155/2018/8430614

U2 - 10.1155/2018/8430614

DO - 10.1155/2018/8430614

M3 - Article

C2 - 29670469

SN - 0962-9351

VL - 2018

JO - Mediators of Inflammation

JF - Mediators of Inflammation

M1 - 8430614

ER -

Dietary n-3 PUFA May Attenuate Experimental Colitis

Abstract

Keywords

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