Diagnostic accuracy and clinical utility of a simplified low cos method of counting CD4 cells with flow cytometry in Malawi diagnostic accuracy study

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Diagnostic accuracy and clinical utility of a simplified low cos method of counting CD4 cells with flow cytometry in Malawi diagnostic accuracy study. / MacLennan, Calman; Liu, MKP; White, SA; van Osterhout, JJG; Simukonda, F; Bwanali, J; Moore, MJ; Zijlstra, EE; Drayson, Mark; Molyneux, ME.

In: British Medical Journal (International edition), Vol. 335, No. 7612, 01.07.2007, p. 190-194.

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MacLennan, Calman ; Liu, MKP ; White, SA ; van Osterhout, JJG ; Simukonda, F ; Bwanali, J ; Moore, MJ ; Zijlstra, EE ; Drayson, Mark ; Molyneux, ME. / Diagnostic accuracy and clinical utility of a simplified low cos method of counting CD4 cells with flow cytometry in Malawi diagnostic accuracy study. In: British Medical Journal (International edition). 2007 ; Vol. 335, No. 7612. pp. 190-194.

Bibtex

@article{fd622f8575f24479994598d7b3b53204,
title = "Diagnostic accuracy and clinical utility of a simplified low cos method of counting CD4 cells with flow cytometry in Malawi diagnostic accuracy study",
abstract = "Objectives : To assess the diagnostic accuracy and clinical utility of a simplified low cost method for measuring absolute and percentage CD4 counts with flow cytometry. Design : A CD4 counting method (Blantyre count) using a CD4 and CD45 antibody combination with reduced blood and reagent volumes. Diagnostic accuracy was assessed by measuring agreement of the index test with two other assays (TruCount and FACSCount). Clinical utility was investigated by comparing CD4 counts with the new assay with WHO clinical staging in patients with HIV. Setting : Research laboratories and antiretroviral therapy clinic at a medical school and large government hospital in southern Malawi. Participants : Assay comparisons were performed on consecutive blood samples sent for CD4 counting from 129 patients with HIV. Comparison of CD4 count with staging was conducted on 253 consecutive new patients attending the antiretroviral therapy clinic. Main outcome measures : Limits of agreement with 95% confidence intervals between index test and reference standards. Results : The limits of agreement for Blantyre count and TruCount were excellent (cell count −48.9 to 27.0 cells/μl for absolute counts in the CD4 range 350 cells/μl. Conclusions : This simplified method of counting CD4 cells with flow cytometry has good agreement with established commercial assays, is affordable for routine clinical use in Africa, and could improve clinical decision making in patients with HIV.",
author = "Calman MacLennan and MKP Liu and SA White and {van Osterhout}, JJG and F Simukonda and J Bwanali and MJ Moore and EE Zijlstra and Mark Drayson and ME Molyneux",
year = "2007",
month = jul,
day = "1",
doi = "10.1136/bmj.39268.719780.BE",
language = "English",
volume = "335",
pages = "190--194",
journal = "British Medical Journal (International edition)",
issn = "0959-8146",
publisher = "BMJ Publishing Group",
number = "7612",

}

RIS

TY - JOUR

T1 - Diagnostic accuracy and clinical utility of a simplified low cos method of counting CD4 cells with flow cytometry in Malawi diagnostic accuracy study

AU - MacLennan, Calman

AU - Liu, MKP

AU - White, SA

AU - van Osterhout, JJG

AU - Simukonda, F

AU - Bwanali, J

AU - Moore, MJ

AU - Zijlstra, EE

AU - Drayson, Mark

AU - Molyneux, ME

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Objectives : To assess the diagnostic accuracy and clinical utility of a simplified low cost method for measuring absolute and percentage CD4 counts with flow cytometry. Design : A CD4 counting method (Blantyre count) using a CD4 and CD45 antibody combination with reduced blood and reagent volumes. Diagnostic accuracy was assessed by measuring agreement of the index test with two other assays (TruCount and FACSCount). Clinical utility was investigated by comparing CD4 counts with the new assay with WHO clinical staging in patients with HIV. Setting : Research laboratories and antiretroviral therapy clinic at a medical school and large government hospital in southern Malawi. Participants : Assay comparisons were performed on consecutive blood samples sent for CD4 counting from 129 patients with HIV. Comparison of CD4 count with staging was conducted on 253 consecutive new patients attending the antiretroviral therapy clinic. Main outcome measures : Limits of agreement with 95% confidence intervals between index test and reference standards. Results : The limits of agreement for Blantyre count and TruCount were excellent (cell count −48.9 to 27.0 cells/μl for absolute counts in the CD4 range 350 cells/μl. Conclusions : This simplified method of counting CD4 cells with flow cytometry has good agreement with established commercial assays, is affordable for routine clinical use in Africa, and could improve clinical decision making in patients with HIV.

AB - Objectives : To assess the diagnostic accuracy and clinical utility of a simplified low cost method for measuring absolute and percentage CD4 counts with flow cytometry. Design : A CD4 counting method (Blantyre count) using a CD4 and CD45 antibody combination with reduced blood and reagent volumes. Diagnostic accuracy was assessed by measuring agreement of the index test with two other assays (TruCount and FACSCount). Clinical utility was investigated by comparing CD4 counts with the new assay with WHO clinical staging in patients with HIV. Setting : Research laboratories and antiretroviral therapy clinic at a medical school and large government hospital in southern Malawi. Participants : Assay comparisons were performed on consecutive blood samples sent for CD4 counting from 129 patients with HIV. Comparison of CD4 count with staging was conducted on 253 consecutive new patients attending the antiretroviral therapy clinic. Main outcome measures : Limits of agreement with 95% confidence intervals between index test and reference standards. Results : The limits of agreement for Blantyre count and TruCount were excellent (cell count −48.9 to 27.0 cells/μl for absolute counts in the CD4 range 350 cells/μl. Conclusions : This simplified method of counting CD4 cells with flow cytometry has good agreement with established commercial assays, is affordable for routine clinical use in Africa, and could improve clinical decision making in patients with HIV.

U2 - 10.1136/bmj.39268.719780.BE

DO - 10.1136/bmj.39268.719780.BE

M3 - Article

VL - 335

SP - 190

EP - 194

JO - British Medical Journal (International edition)

JF - British Medical Journal (International edition)

SN - 0959-8146

IS - 7612

ER -