Development of functional thymic epithelial cells occurs independently of lymphostromal interactions.

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@article{d5060336a26a4bc48e56b1e07717b664,
title = "Development of functional thymic epithelial cells occurs independently of lymphostromal interactions.",
abstract = "The thymus provides a specialised microenvironment for the development of T-cell precursors. This developmental programme depends upon interactions with stromal cells such as thymic epithelial cells, which provide signals for proliferation, survival and differentiation. In turn, it has been proposed that development of thymic epithelial cells themselves is regulated by signals produced by developing thymocytes. Evidence in support of this symbiotic relationship, termed thymic crosstalk, comes from studies analysing the thymus of adult mice harbouring blocks at specific stages of thymocyte development, where it is difficult to separate mechanisms regulating the initial development of thymic epithelial cells from those regulating their maintenance. To distinguish between these processes, we have analysed the initial developmental programme of thymic epithelial cells within the embryonic thymus, in either the presence or absence of normal T-cell development. We show that keratin 5+8+ precursor epithelial cells present in the early thymic rudiment differentiate into discrete cortical and medullary epithelial subsets displaying normal gene expression profiles, and acquire functional competence, independently of signals from T-cell precursors. Thus, our findings redefine current models of thymus development and argue against a role for thymocyte-epithelial cell crosstalk in the development of thymic epithelial progenitors.",
keywords = "thymic epithelium, T-cell, differentiation, thymus",
author = "William Jenkinson and Simona Rossi and Eric Jenkinson and Graham Anderson",
year = "2005",
month = dec,
day = "1",
doi = "10.1016/j.mod.2005.08.003",
language = "English",
volume = "122",
pages = "1294--9",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - Development of functional thymic epithelial cells occurs independently of lymphostromal interactions.

AU - Jenkinson, William

AU - Rossi, Simona

AU - Jenkinson, Eric

AU - Anderson, Graham

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The thymus provides a specialised microenvironment for the development of T-cell precursors. This developmental programme depends upon interactions with stromal cells such as thymic epithelial cells, which provide signals for proliferation, survival and differentiation. In turn, it has been proposed that development of thymic epithelial cells themselves is regulated by signals produced by developing thymocytes. Evidence in support of this symbiotic relationship, termed thymic crosstalk, comes from studies analysing the thymus of adult mice harbouring blocks at specific stages of thymocyte development, where it is difficult to separate mechanisms regulating the initial development of thymic epithelial cells from those regulating their maintenance. To distinguish between these processes, we have analysed the initial developmental programme of thymic epithelial cells within the embryonic thymus, in either the presence or absence of normal T-cell development. We show that keratin 5+8+ precursor epithelial cells present in the early thymic rudiment differentiate into discrete cortical and medullary epithelial subsets displaying normal gene expression profiles, and acquire functional competence, independently of signals from T-cell precursors. Thus, our findings redefine current models of thymus development and argue against a role for thymocyte-epithelial cell crosstalk in the development of thymic epithelial progenitors.

AB - The thymus provides a specialised microenvironment for the development of T-cell precursors. This developmental programme depends upon interactions with stromal cells such as thymic epithelial cells, which provide signals for proliferation, survival and differentiation. In turn, it has been proposed that development of thymic epithelial cells themselves is regulated by signals produced by developing thymocytes. Evidence in support of this symbiotic relationship, termed thymic crosstalk, comes from studies analysing the thymus of adult mice harbouring blocks at specific stages of thymocyte development, where it is difficult to separate mechanisms regulating the initial development of thymic epithelial cells from those regulating their maintenance. To distinguish between these processes, we have analysed the initial developmental programme of thymic epithelial cells within the embryonic thymus, in either the presence or absence of normal T-cell development. We show that keratin 5+8+ precursor epithelial cells present in the early thymic rudiment differentiate into discrete cortical and medullary epithelial subsets displaying normal gene expression profiles, and acquire functional competence, independently of signals from T-cell precursors. Thus, our findings redefine current models of thymus development and argue against a role for thymocyte-epithelial cell crosstalk in the development of thymic epithelial progenitors.

KW - thymic epithelium

KW - T-cell

KW - differentiation

KW - thymus

U2 - 10.1016/j.mod.2005.08.003

DO - 10.1016/j.mod.2005.08.003

M3 - Article

C2 - 16274965

VL - 122

SP - 1294

EP - 1299

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 12

ER -