Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

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Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. / Bilverstone, T W; Kinsmore, N L; Minton, N P; Kuehne, Sarah.

In: Anaerobe, Vol. 44, 04.2017, p. 51-54.

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@article{4e47ec35f2ae4b20a7f7a3bdb1226b47,
title = "Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels",
abstract = "Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.",
keywords = "ADP Ribose Transferases, Animals, Bacterial Proteins, Cell Survival, Cercopithecus aethiops, Clostridium difficile, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Regulator, Vero Cells, Journal Article",
author = "Bilverstone, {T W} and Kinsmore, {N L} and Minton, {N P} and Sarah Kuehne",
note = "Copyright {\textcopyright} 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.",
year = "2017",
month = apr,
doi = "10.1016/j.anaerobe.2017.01.009",
language = "English",
volume = "44",
pages = "51--54",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

AU - Bilverstone, T W

AU - Kinsmore, N L

AU - Minton, N P

AU - Kuehne, Sarah

N1 - Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2017/4

Y1 - 2017/4

N2 - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

AB - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

KW - ADP Ribose Transferases

KW - Animals

KW - Bacterial Proteins

KW - Cell Survival

KW - Cercopithecus aethiops

KW - Clostridium difficile

KW - Gene Deletion

KW - Gene Expression Regulation, Bacterial

KW - Genes, Regulator

KW - Vero Cells

KW - Journal Article

U2 - 10.1016/j.anaerobe.2017.01.009

DO - 10.1016/j.anaerobe.2017.01.009

M3 - Article

C2 - 28108389

VL - 44

SP - 51

EP - 54

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -