Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Research output: Contribution to journalArticlepeer-review


Colleges, School and Institutes

External organisations

  • University of Nottingham
  • Nottingham University Hospitals NHS Trust


Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.


Original languageEnglish
Pages (from-to)51-54
Number of pages4
Early online date17 Jan 2017
Publication statusPublished - Apr 2017


  • ADP Ribose Transferases, Animals, Bacterial Proteins, Cell Survival, Cercopithecus aethiops, Clostridium difficile, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Regulator, Vero Cells, Journal Article