Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
Research output: Contribution to journal › Article › peer-review
We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.
|Journal||Journal of Bioscience and Bioengineering|
|Early online date||2 Jul 2015|
|Publication status||Published - 31 Jan 2016|
- Polyhydroxyalkanoate , Dimethyl sulphoxide , Solvent extraction , Bioplastic