Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Isaac Vizcaino Caston; Catherine Kelly; Annabel Fitzgerald; Gary Leeke; Michael Jenkins; Timothy Overton

doi:10.1016/j.jbiosc.2015.04.021

Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Isaac Vizcaino Caston, Catherine Kelly, Annabel Fitzgerald, Gary Leeke, Michael Jenkins, Timothy Overton

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Abstract

We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.

Original language	English
Pages (from-to)	101-104
Journal	Journal of Bioscience and Bioengineering
Volume	121
Issue number	1
Early online date	2 Jul 2015
DOIs	https://doi.org/10.1016/j.jbiosc.2015.04.021
Publication status	Published - 31 Jan 2016

Keywords

Polyhydroxyalkanoate
Dimethyl sulphoxide
Solvent extraction
Bioplastic

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10.1016/j.jbiosc.2015.04.021Licence: None: All rights reserved

Vizcaino-Caston_et_al_Development_Rapid_Method_Bioscience_and _Bioengineering_2015_Post-Print
NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Bioscience and Bioengineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Bioscience and Bioengineering, DOI: 10.1016/j.jbiosc.2015.04.021. After an embargo period this document can be shared subject to the terms of a Creative Commons Attribution Non-Commercial No-Derivatives license. Eligibility for repository checked July 2015
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Cite this

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title = "Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies",

abstract = "We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.",

keywords = "Polyhydroxyalkanoate , Dimethyl sulphoxide , Solvent extraction , Bioplastic",

author = "{Vizcaino Caston}, Isaac and Catherine Kelly and Annabel Fitzgerald and Gary Leeke and Michael Jenkins and Timothy Overton",

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AU - Vizcaino Caston, Isaac

AU - Kelly, Catherine

AU - Fitzgerald, Annabel

AU - Leeke, Gary

AU - Jenkins, Michael

AU - Overton, Timothy

PY - 2016/1/31

Y1 - 2016/1/31

N2 - We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.

AB - We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using FT-IR spectroscopy and differential scanning calorimetry.

KW - Polyhydroxyalkanoate

KW - Dimethyl sulphoxide

KW - Solvent extraction

KW - Bioplastic

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DO - 10.1016/j.jbiosc.2015.04.021

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EP - 104

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 1

ER -

Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

Abstract

Keywords

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