Development and application of LED arrays for use in phototherapy research

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@article{38ea10a7ecc948e2ae7a3b13a389add1,
title = "Development and application of LED arrays for use in phototherapy research",
abstract = "Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies.96-well plate format LED arrays (400–850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5–142 mW/cm2: 15–120 s) and the biological responses were assessed using MTT assays.Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm2 at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48–142 mW/cm2).LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.",
keywords = "photobiomodulation, phototherapy, photodisinfection, dentine-pulp complex, stem cells, low level light therapy",
author = "Mohammed Hadis and Paul Cooper and Michael Milward and Patricia Gorecki and Edward Tarte and William Palin and James Churm",
year = "2017",
month = feb
day = "7",
doi = "10.1002/jbio.201600273",
language = "English",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

}

RIS

TY - JOUR

T1 - Development and application of LED arrays for use in phototherapy research

AU - Hadis, Mohammed

AU - Cooper, Paul

AU - Milward, Michael

AU - Gorecki, Patricia

AU - Tarte, Edward

AU - Palin, William

AU - Churm, James

PY - 2017/2/7

Y1 - 2017/2/7

N2 - Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies.96-well plate format LED arrays (400–850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5–142 mW/cm2: 15–120 s) and the biological responses were assessed using MTT assays.Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm2 at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48–142 mW/cm2).LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.

AB - Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies.96-well plate format LED arrays (400–850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5–142 mW/cm2: 15–120 s) and the biological responses were assessed using MTT assays.Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm2 at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48–142 mW/cm2).LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.

KW - photobiomodulation

KW - phototherapy

KW - photodisinfection

KW - dentine-pulp complex

KW - stem cells

KW - low level light therapy

U2 - 10.1002/jbio.201600273

DO - 10.1002/jbio.201600273

M3 - Article

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

ER -