Determination of the extracellular glycine concentration in the rat spinal cord dorsal horn by quantitative microdialysis
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Two quantitative microdialysis methods were used to determine the extracellular concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats. Extracellular glycine determined by zero net flux was 2.6+/-0.3 microM and by the zero flow method was 3.3+/-0.3 microM. For comparison the glycine content of cerebrospinal fluid was determined to be 6.4+/-1.1 microM. There was no correlation between the extracellular and the cerebrospinal fluid concentrations.
|Number of pages||3|
|Publication status||Published - 10 Aug 2001|
- extracellular concentration, quantitative microdialysis, glycine, spinal cord dorsal horn, glycine transporter