Detection of tobacco mosaic tobamovirus in cigarettes through RT-PCR

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Detection of tobacco mosaic tobamovirus in cigarettes through RT-PCR. / Iftikhar, Yasir; Jackson, Robert; Neuman, Benjamin W.

In: Pakistan Journal of Agricultural Sciences, Vol. 52, No. 3, 09.2015, p. 667-670.

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@article{4815e8ef72444976802b32bda5ad829a,
title = "Detection of tobacco mosaic tobamovirus in cigarettes through RT-PCR",
abstract = "TMV was tried to recover from a variety of branded cigarettes and cigars. Tobacco from six different brands of cigarettes and cigar were processed and reverse transcriptase polymerase chain reaction was employed for the detection of TMV. RTPCR confirmed the presence of TMV in tobacco from one brand of cigarette and one brand of cigar. Bean plants (Phaseolus vulgaris) were inoculated manually with tobacco sap of cigarettes resulting in the production of localized disease lesions. Together, these results showed that tobacco used to make cigarettes and cigars can function as an effective disease vector, potentially aiding the movement of infectious TMV between countries. This is an important finding prompting a need to test smoking tobacco for other virus particles that infect tobacco plants and survive processing as well as considering biosecurity measures to limit virus transmission.",
keywords = "Electrophoresis, Indexing, Smoking tobacco, TMV, Virus transmission",
author = "Yasir Iftikhar and Robert Jackson and Neuman, {Benjamin W.}",
year = "2015",
month = sep,
language = "English",
volume = "52",
pages = "667--670",
journal = "Pakistan Journal of Agricultural Sciences",
issn = "0552-9034",
publisher = "Pakistan Association of Advancement in Agricultural Sciences",
number = "3",

}

RIS

TY - JOUR

T1 - Detection of tobacco mosaic tobamovirus in cigarettes through RT-PCR

AU - Iftikhar, Yasir

AU - Jackson, Robert

AU - Neuman, Benjamin W.

PY - 2015/9

Y1 - 2015/9

N2 - TMV was tried to recover from a variety of branded cigarettes and cigars. Tobacco from six different brands of cigarettes and cigar were processed and reverse transcriptase polymerase chain reaction was employed for the detection of TMV. RTPCR confirmed the presence of TMV in tobacco from one brand of cigarette and one brand of cigar. Bean plants (Phaseolus vulgaris) were inoculated manually with tobacco sap of cigarettes resulting in the production of localized disease lesions. Together, these results showed that tobacco used to make cigarettes and cigars can function as an effective disease vector, potentially aiding the movement of infectious TMV between countries. This is an important finding prompting a need to test smoking tobacco for other virus particles that infect tobacco plants and survive processing as well as considering biosecurity measures to limit virus transmission.

AB - TMV was tried to recover from a variety of branded cigarettes and cigars. Tobacco from six different brands of cigarettes and cigar were processed and reverse transcriptase polymerase chain reaction was employed for the detection of TMV. RTPCR confirmed the presence of TMV in tobacco from one brand of cigarette and one brand of cigar. Bean plants (Phaseolus vulgaris) were inoculated manually with tobacco sap of cigarettes resulting in the production of localized disease lesions. Together, these results showed that tobacco used to make cigarettes and cigars can function as an effective disease vector, potentially aiding the movement of infectious TMV between countries. This is an important finding prompting a need to test smoking tobacco for other virus particles that infect tobacco plants and survive processing as well as considering biosecurity measures to limit virus transmission.

KW - Electrophoresis

KW - Indexing

KW - Smoking tobacco

KW - TMV

KW - Virus transmission

UR - http://www.scopus.com/inward/record.url?scp=84944053112&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84944053112

VL - 52

SP - 667

EP - 670

JO - Pakistan Journal of Agricultural Sciences

JF - Pakistan Journal of Agricultural Sciences

SN - 0552-9034

IS - 3

ER -