Detection of tobacco mosaic tobamovirus in cigarettes through RT-PCR

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

External organisations

  • University of Sargodha
  • Reading University

Abstract

TMV was tried to recover from a variety of branded cigarettes and cigars. Tobacco from six different brands of cigarettes and cigar were processed and reverse transcriptase polymerase chain reaction was employed for the detection of TMV. RTPCR confirmed the presence of TMV in tobacco from one brand of cigarette and one brand of cigar. Bean plants (Phaseolus vulgaris) were inoculated manually with tobacco sap of cigarettes resulting in the production of localized disease lesions. Together, these results showed that tobacco used to make cigarettes and cigars can function as an effective disease vector, potentially aiding the movement of infectious TMV between countries. This is an important finding prompting a need to test smoking tobacco for other virus particles that infect tobacco plants and survive processing as well as considering biosecurity measures to limit virus transmission.

Details

Original languageEnglish
Pages (from-to)667-670
Number of pages4
JournalPakistan Journal of Agricultural Sciences
Volume52
Issue number3
Publication statusPublished - Sep 2015

Keywords

  • Electrophoresis, Indexing, Smoking tobacco, TMV, Virus transmission