Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

Research output: Contribution to journalArticle

Authors

  • ZY Zhao
  • M San
  • JL Duprey
  • JS Vyle

Colleges, School and Institutes

Abstract

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.

Details

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalBioorganic & Medicinal Chemistry Letters
Volume22
Issue number1
Publication statusPublished - 1 Jan 2012

Keywords

  • DNA, SNP, Fluorescence, Sensing, Nucleic acids