Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

ZY Zhao, M San, JL Duprey, John Arrand, JS Vyle, James Tucker

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.
Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalBioorganic & Medicinal Chemistry Letters
Volume22
Issue number1
DOIs
Publication statusPublished - 1 Jan 2012

Keywords

  • DNA
  • SNP
  • Fluorescence
  • Sensing
  • Nucleic acids

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