Detecting gene expression in lymphoid microenvironments by laser microdissection and quantitative RT-PCR

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Authors

Colleges, School and Institutes

Abstract

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is a valuable tool for measuring gene expression in cells and tissues. Unique challenges are encountered when studies are performed on cells microdissected from small specific areas of frozen animal or human tissue. This chapter describes the analysis of gene expression of chemokines and cytokines that are important for the differentiation and migration of germinal center (GC) derived plasmablasts/plasma cells and memory B cells by using laser capture microdissection (LCM) and qRT-PCR to examine tissue sections.

Bibliographic note

Methods in Molecular Biology is out of scope for Ref

Details

Original languageEnglish
Title of host publicationGerminal Centers
Subtitle of host publicationMethods and Protocols
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1623

Keywords

  • Quantitative RT-PCR , Laser capture microdissection , Germinal center , B cells