Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability

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Dendrimer mediated clustering of bacteria : improved aggregation and evaluation of bacterial response and viability. / Leire, Emma; Amaral, Sandra P; Louzao, Iria; Winzer, Klaus; Alexander, Cameron; Fernandez-Megia, Eduardo; Fernandez-Trillo, Francisco.

In: Biomaterials Science, 29.04.2016.

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Leire, Emma ; Amaral, Sandra P ; Louzao, Iria ; Winzer, Klaus ; Alexander, Cameron ; Fernandez-Megia, Eduardo ; Fernandez-Trillo, Francisco. / Dendrimer mediated clustering of bacteria : improved aggregation and evaluation of bacterial response and viability. In: Biomaterials Science. 2016.

Bibtex

@article{15fe6c8ce0e4466fb24e38cb7ec83651,
title = "Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability",
abstract = "Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can induce the formation of clusters in Vibrio harveyi, an opportunistic marine pathogen, in a generation dependent manner. Moreover, these cationic GATG dendrimers demonstrate an improved ability to induce cluster formation when compared to poly(N-[3-(dimethylamino)propyl]methacrylamide) [p(DMAPMAm)], a cationic linear polymer previously shown to cluster bacteria. Viability of the bacteria within the formed clusters and evaluation of quorum sensing controlled phenotypes (i.e. light production in V. harveyi) suggest that GATG dendrimers may be activating microbial responses by maintaining a high concentration of quorum sensing signals inside the clusters while increasing permeability of the microbial outer membranes. Thus, the reported GATG dendrimers constitute a valuable platform for the development of novel antimicrobial materials that can target microbial viability and/or virulence.",
author = "Emma Leire and Amaral, {Sandra P} and Iria Louzao and Klaus Winzer and Cameron Alexander and Eduardo Fernandez-Megia and Francisco Fernandez-Trillo",
year = "2016",
month = apr,
day = "29",
doi = "10.1039/c6bm00079g",
language = "English",
journal = "Biomaterials Science",
issn = "2047-4830",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Dendrimer mediated clustering of bacteria

T2 - improved aggregation and evaluation of bacterial response and viability

AU - Leire, Emma

AU - Amaral, Sandra P

AU - Louzao, Iria

AU - Winzer, Klaus

AU - Alexander, Cameron

AU - Fernandez-Megia, Eduardo

AU - Fernandez-Trillo, Francisco

PY - 2016/4/29

Y1 - 2016/4/29

N2 - Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can induce the formation of clusters in Vibrio harveyi, an opportunistic marine pathogen, in a generation dependent manner. Moreover, these cationic GATG dendrimers demonstrate an improved ability to induce cluster formation when compared to poly(N-[3-(dimethylamino)propyl]methacrylamide) [p(DMAPMAm)], a cationic linear polymer previously shown to cluster bacteria. Viability of the bacteria within the formed clusters and evaluation of quorum sensing controlled phenotypes (i.e. light production in V. harveyi) suggest that GATG dendrimers may be activating microbial responses by maintaining a high concentration of quorum sensing signals inside the clusters while increasing permeability of the microbial outer membranes. Thus, the reported GATG dendrimers constitute a valuable platform for the development of novel antimicrobial materials that can target microbial viability and/or virulence.

AB - Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can induce the formation of clusters in Vibrio harveyi, an opportunistic marine pathogen, in a generation dependent manner. Moreover, these cationic GATG dendrimers demonstrate an improved ability to induce cluster formation when compared to poly(N-[3-(dimethylamino)propyl]methacrylamide) [p(DMAPMAm)], a cationic linear polymer previously shown to cluster bacteria. Viability of the bacteria within the formed clusters and evaluation of quorum sensing controlled phenotypes (i.e. light production in V. harveyi) suggest that GATG dendrimers may be activating microbial responses by maintaining a high concentration of quorum sensing signals inside the clusters while increasing permeability of the microbial outer membranes. Thus, the reported GATG dendrimers constitute a valuable platform for the development of novel antimicrobial materials that can target microbial viability and/or virulence.

U2 - 10.1039/c6bm00079g

DO - 10.1039/c6bm00079g

M3 - Article

C2 - 27127812

JO - Biomaterials Science

JF - Biomaterials Science

SN - 2047-4830

ER -