De novo design of Ln(III) coiled coils for imaging applications

Matthew R. Berwick; David J. Lewis; Andrew W. Jones; Rosemary A. Parslow; Timothy R. Dafforn; Helen J. Cooper; John Wilkie; Zoe Pikramenou; Melanie M. Britton; Anna F. A. Peacock

doi:10.1021/ja408741h

De novo design of Ln(III) coiled coils for imaging applications

Matthew R. Berwick, David J. Lewis, Andrew W. Jones, Rosemary A. Parslow, Timothy R. Dafforn, Helen J. Cooper, John Wilkie, Zoe Pikramenou, Melanie M. Britton, Anna F. A. Peacock

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40 Citations (Scopus)

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Abstract

A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.

Original language	English
Pages (from-to)	1166-1169
Journal	Journal of the American Chemical Society
Volume	136
Issue number	4
Early online date	9 Jan 2014
DOIs	https://doi.org/10.1021/ja408741h https://doi.org/10.1021/ja408741h
Publication status	Published - 29 Jan 2014

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Luminescent nanoparticles as trackers for imaging of flows and sensing phenomena in microchannels
Pikramenou, Z.
Engineering & Physical Science Research Council
18/05/09 → 17/01/13
Project: Research Councils

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Berwick, M. R., Lewis, D. J., Jones, A. W., Parslow, R. A., Dafforn, T. R., Cooper, H. J., Wilkie, J., Pikramenou, Z., Britton, M. M., & Peacock, A. F. A. (2014). De novo design of Ln(III) coiled coils for imaging applications. Journal of the American Chemical Society, 136(4), 1166-1169. Advance online publication. https://doi.org/10.1021/ja408741h, https://doi.org/10.1021/ja408741h

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abstract = "A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.",

author = "Berwick, {Matthew R.} and Lewis, {David J.} and Jones, {Andrew W.} and Parslow, {Rosemary A.} and Dafforn, {Timothy R.} and Cooper, {Helen J.} and John Wilkie and Zoe Pikramenou and Britton, {Melanie M.} and Peacock, {Anna F. A.}",

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AU - Dafforn, Timothy R.

AU - Cooper, Helen J.

AU - Wilkie, John

AU - Pikramenou, Zoe

AU - Britton, Melanie M.

AU - Peacock, Anna F. A.

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De novo design of Ln(III) coiled coils for imaging applications

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Luminescent nanoparticles as trackers for imaging of flows and sensing phenomena in microchannels

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