Data comparing the separation and elution of vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometer (UPSFC-MS/MS) compared to liquid chromatography (LC) and data presenting approaches to UPSFC method optimization

Research output: Contribution to journalArticle

Colleges, School and Institutes

Abstract

The data presented is related to the research article “Analysis of multiple vitamin D metabolites by ultra performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS)” (Jenkinson et al., 2018) [1]. This article will include data obtained from method development, optimization and analysis of multiple vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometry (UPSFC-MS/MS). This includes chromatograms from column screening to confirm the most suitable column for analyte separation. Additionally, further chromatograms and figures compare separation and analyte signal strength during the optimization of other UPSFC parameters. Mass spectra will demonstrate the optimization of MS conditions for the UPSFC-MS/MS method. Chromatogram data from UHPLC vitamin D analysis is also presented in order to compare the separation and elution of vitamin D metabolites using UPSFC and UHPLC. This data will highlight the outputs that aid in method development and identifying the separation technique suited for vitamin D quantitation.

Details

Original languageEnglish
Pages (from-to)426-435
JournalData in Brief
Volume20
Early online date15 Aug 2018
Publication statusPublished - 1 Oct 2018

Keywords

  • vitamin D, method development, UPSFC-MS/MS, LC-MS/MS