Cytological analysis of Arabidopsis thaliana meiotic chromosomes

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Abstract

Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.

Details

Original languageEnglish
Pages (from-to)131-45
Number of pages15
JournalMethods in molecular biology
Volume558
Publication statusPublished - 1 Jan 2009