Cytological analysis of Arabidopsis thaliana meiotic chromosomes

Susan Armstrong; Eugenio Sanchez-Moran; Frederick Franklin

doi:10.1007/978-1-60761-103-5_9

Cytological analysis of Arabidopsis thaliana meiotic chromosomes

Susan Armstrong, Eugenio Sanchez-Moran, Frederick Franklin

Biosciences

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Abstract

Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.

Original language	English
Pages (from-to)	131-45
Number of pages	15
Journal	Methods in molecular biology
Volume	558
DOIs	https://doi.org/10.1007/978-1-60761-103-5_9
Publication status	Published - 1 Jan 2009

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10.1007/978-1-60761-103-5_9

Integrating chromatin structure and global chromosome dynamics
Sanchez-Moran, E.
Biotechnology & Biological Sciences Research Council
1/10/08 → 28/07/14
Project: Research Councils
Integration of chromosome synapsis and recombination by AtZYP1 during Arabidopsis meiosis
Franklin, C., Jones, G. & Armstrong, S.
Biotechnology & Biological Sciences Research Council
1/01/07 → 30/04/10
Project: Research Councils

Cite this

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title = "Cytological analysis of Arabidopsis thaliana meiotic chromosomes",

abstract = "Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.",

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AB - Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.

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JO - Methods in molecular biology

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Cytological analysis of Arabidopsis thaliana meiotic chromosomes

Abstract

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Projects

Integrating chromatin structure and global chromosome dynamics

Integration of chromosome synapsis and recombination by AtZYP1 during Arabidopsis meiosis

Cite this