TY - JOUR
T1 - Cytokine and chemokine levels in tears and in corneal fibroblast cultures before and after excimer laser treatment
AU - Leonardi, A
AU - Tavolato, M
AU - Curnow, Stephen
AU - Fregona, I
AU - Violato, D
AU - Alio, J
PY - 2009/2/1
Y1 - 2009/2/1
N2 - PURPOSE: To measure multiple cytokine and chemokine production in tears of myopic patients before and after laser in situ keratomileusis (LASIK) and in human corneal fibroblast (HCF) cultures before and after excimer laser treatment. SETTING: Department of Neuroscience, Ophthalmology Unit, University of Padua, Italy and Vissum-Instituto de Oftalmológico de Alicante, Alicante, Spain METHODS: Tear samples were obtained from 15 myopic patients before LASIK and 1 and 24 hours after LASIK. Quiescent HCF cultures were treated using the same laser energy. Culture medium was collected before treatment and after 1 and 24 hours. Cytokine concentrations were determined using multiplexed bead analysis. RESULTS: Compared with baseline values, interleukin (IL)-12 tear levels were significantly increased 1 hour after surgery and eotaxin levels were significantly increased at 24 hours (both P
AB - PURPOSE: To measure multiple cytokine and chemokine production in tears of myopic patients before and after laser in situ keratomileusis (LASIK) and in human corneal fibroblast (HCF) cultures before and after excimer laser treatment. SETTING: Department of Neuroscience, Ophthalmology Unit, University of Padua, Italy and Vissum-Instituto de Oftalmológico de Alicante, Alicante, Spain METHODS: Tear samples were obtained from 15 myopic patients before LASIK and 1 and 24 hours after LASIK. Quiescent HCF cultures were treated using the same laser energy. Culture medium was collected before treatment and after 1 and 24 hours. Cytokine concentrations were determined using multiplexed bead analysis. RESULTS: Compared with baseline values, interleukin (IL)-12 tear levels were significantly increased 1 hour after surgery and eotaxin levels were significantly increased at 24 hours (both P
U2 - 10.1016/j.jcrs.2008.10.030
DO - 10.1016/j.jcrs.2008.10.030
M3 - Article
C2 - 19185237
VL - 35
SP - 240
EP - 247
JO - Journal of cataract and refractive surgery
JF - Journal of cataract and refractive surgery
IS - 2
ER -