Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance

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Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance. / Marquez, Diego; Stuart-Williams, Hilary; Farquhar, Graham D.; Busch, Florian.

In: New Phytologist, 30.06.2021.

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@article{e32275bc24004ac6858c61f582e5e3ff,
title = "Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance",
abstract = "Cuticular conductance to water (g cw) is difficult to quantify for stomatous surfaces due to the complexity of separating cuticular and stomatal transpiration, and additional complications arise for determining adaxial and abaxial g cw. This has led to the neglect of g cw as a separate parameter in most common gas exchange measurements. Here, we describe a simple technique to simultaneously estimate adaxial and abaxial values of g cw, tested in two amphistomatous plant species. What we term the {\textquoteleft}Red-Light method{\textquoteright} is used to estimate g cw from gas exchange measurements and a known CO 2 concentration inside the leaf during photosynthetic induction under red light. We provide an easy-to-use web application to assist with the calculation of g cw. While adaxial and abaxial g cw varies significantly between leaves of the same species we found that the ratio of adaxial/abaxial g cw (γ n) is stable within a plant species. This has implications for use of generic values of g cw when analysing gas exchange data. The Red-Light method can be used to estimate total cuticular conductance (g cw-T) accurately with the most common setup of gas exchange instruments, i.e. a chamber mixing the adaxial and abaxial gases, allowing for a wide application of this technique. ",
keywords = "abaxial cuticular conductance, adaxial cuticular conductance, amphistomatous leaf, cuticular conductance to water, leaf gas exchange, nocturnal conductance, photosynthetic induction",
author = "Diego Marquez and Hilary Stuart-Williams and Farquhar, {Graham D.} and Florian Busch",
note = "Funding Information: The authors thank CONICYT, Doctorado Becas Chile/2015 Folio: 72160160 and the ARC Centre of Excellence for Translational Photosynthesis for funding part of the research; Suan Chin Wong for providing technical support; the Centre for Advanced Microscopy at the Australian National University (ANU) for instruments and technical assistance; ANU Plant Services for taking care of the plant material. Publisher Copyright: {\textcopyright} 2021 The Authors New Phytologist {\textcopyright} 2021 New Phytologist Foundation",
year = "2021",
month = jun,
day = "30",
doi = "10.1111/nph.17588",
language = "English",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",

}

RIS

TY - JOUR

T1 - Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance

AU - Marquez, Diego

AU - Stuart-Williams, Hilary

AU - Farquhar, Graham D.

AU - Busch, Florian

N1 - Funding Information: The authors thank CONICYT, Doctorado Becas Chile/2015 Folio: 72160160 and the ARC Centre of Excellence for Translational Photosynthesis for funding part of the research; Suan Chin Wong for providing technical support; the Centre for Advanced Microscopy at the Australian National University (ANU) for instruments and technical assistance; ANU Plant Services for taking care of the plant material. Publisher Copyright: © 2021 The Authors New Phytologist © 2021 New Phytologist Foundation

PY - 2021/6/30

Y1 - 2021/6/30

N2 - Cuticular conductance to water (g cw) is difficult to quantify for stomatous surfaces due to the complexity of separating cuticular and stomatal transpiration, and additional complications arise for determining adaxial and abaxial g cw. This has led to the neglect of g cw as a separate parameter in most common gas exchange measurements. Here, we describe a simple technique to simultaneously estimate adaxial and abaxial values of g cw, tested in two amphistomatous plant species. What we term the ‘Red-Light method’ is used to estimate g cw from gas exchange measurements and a known CO 2 concentration inside the leaf during photosynthetic induction under red light. We provide an easy-to-use web application to assist with the calculation of g cw. While adaxial and abaxial g cw varies significantly between leaves of the same species we found that the ratio of adaxial/abaxial g cw (γ n) is stable within a plant species. This has implications for use of generic values of g cw when analysing gas exchange data. The Red-Light method can be used to estimate total cuticular conductance (g cw-T) accurately with the most common setup of gas exchange instruments, i.e. a chamber mixing the adaxial and abaxial gases, allowing for a wide application of this technique.

AB - Cuticular conductance to water (g cw) is difficult to quantify for stomatous surfaces due to the complexity of separating cuticular and stomatal transpiration, and additional complications arise for determining adaxial and abaxial g cw. This has led to the neglect of g cw as a separate parameter in most common gas exchange measurements. Here, we describe a simple technique to simultaneously estimate adaxial and abaxial values of g cw, tested in two amphistomatous plant species. What we term the ‘Red-Light method’ is used to estimate g cw from gas exchange measurements and a known CO 2 concentration inside the leaf during photosynthetic induction under red light. We provide an easy-to-use web application to assist with the calculation of g cw. While adaxial and abaxial g cw varies significantly between leaves of the same species we found that the ratio of adaxial/abaxial g cw (γ n) is stable within a plant species. This has implications for use of generic values of g cw when analysing gas exchange data. The Red-Light method can be used to estimate total cuticular conductance (g cw-T) accurately with the most common setup of gas exchange instruments, i.e. a chamber mixing the adaxial and abaxial gases, allowing for a wide application of this technique.

KW - abaxial cuticular conductance

KW - adaxial cuticular conductance

KW - amphistomatous leaf

KW - cuticular conductance to water

KW - leaf gas exchange

KW - nocturnal conductance

KW - photosynthetic induction

UR - http://www.scopus.com/inward/record.url?scp=85111019512&partnerID=8YFLogxK

U2 - 10.1111/nph.17588

DO - 10.1111/nph.17588

M3 - Article

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

ER -