Cryptic transmission of ST405 Escherichia coli carrying blaNDM-4 in hospital

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Colleges, School and Institutes

External organisations

  • West China Hospital of Sichuan University
  • State Key Laboratory of Biotherapy


Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying blaNDM-4, an uncommon blaNDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to blaNDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene blaCTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. blaNDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of blaNDM-4. Two copies of IS26 bracketed a 14.6-kb region containing blaNDM-4 and have the potential to form a composite transposon for mediating the mobilization of blaNDM-4.


Original languageEnglish
Article number390
JournalScientific Reports
Publication statusPublished - 10 Jan 2018


  • Antimicrobial resistance, Bacterial Infection

ASJC Scopus subject areas