Critical roles for c-Myb in lymphoid priming and early B-cell development

Research output: Contribution to journalArticle

Authors

  • KT Greig
  • CA de Graaf
  • JM Murphy
  • MR Carpinelli
  • SHM Pang
  • BT Kile
  • DJ Hilton
  • SL Nutt

Abstract

c-Myb is a transcription factor with functions in many hematopoietic lineages. c-Myb-deficient mice display reduced numbers of B cells; however, it is unknown what role c-Myb plays in B lymphopoiesis because no critical target genes have been identified in the B-cell lineage. We demonstrate that conditional deletion of c-Myb in B-cell progenitors completely abolishes B-cell development. c-Myb is required for lymphoid progenitors to respond to the cytokines interleukin-7 and thymic stromal lymphopoietin; in the absence of sufficient c-Myb activity, mice display a B lymphopenia that closely resembles that observed in interleukin-7 receptor alpha-deficient animals. Analysis of the multipotent progenitor compartment indicates that c-Myb is also required for up-regulation of multiple lymphoidassociated genes, including Il7r, and for the subsequent development of the common lymphoid progenitor population. These data show that c-Myb plays a critical role in the regulatory pathways governing lymphoid specification and early B-cell differentiation. (Blood. 2010; 115(14): 2796-2805)

Details

Original languageEnglish
Pages (from-to)2796-2805
Number of pages10
JournalBlood
Volume115
Issue number14
Publication statusPublished - 1 Apr 2010