Cotton-wool-like bioactive glasses for bone regeneration

Research output: Contribution to journalArticle

Standard

Cotton-wool-like bioactive glasses for bone regeneration. / Poologasundarampillai, Gowsihan; Wang, D.; Li, S.; Nakamura, J.; Bradley, R.; Lee, P. D.; Stevens, M. M.; McPhail, David S.; Kasuga, Toshihiro; Jones, J. R.

In: Acta Biomaterialia, Vol. 10, No. 8, 01.08.2014, p. 3733-3746.

Research output: Contribution to journalArticle

Harvard

Poologasundarampillai, G, Wang, D, Li, S, Nakamura, J, Bradley, R, Lee, PD, Stevens, MM, McPhail, DS, Kasuga, T & Jones, JR 2014, 'Cotton-wool-like bioactive glasses for bone regeneration', Acta Biomaterialia, vol. 10, no. 8, pp. 3733-3746. https://doi.org/10.1016/j.actbio.2014.05.020

APA

Poologasundarampillai, G., Wang, D., Li, S., Nakamura, J., Bradley, R., Lee, P. D., ... Jones, J. R. (2014). Cotton-wool-like bioactive glasses for bone regeneration. Acta Biomaterialia, 10(8), 3733-3746. https://doi.org/10.1016/j.actbio.2014.05.020

Vancouver

Author

Poologasundarampillai, Gowsihan ; Wang, D. ; Li, S. ; Nakamura, J. ; Bradley, R. ; Lee, P. D. ; Stevens, M. M. ; McPhail, David S. ; Kasuga, Toshihiro ; Jones, J. R. / Cotton-wool-like bioactive glasses for bone regeneration. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 8. pp. 3733-3746.

Bibtex

@article{34657f988def4594badfe377a19e3a76,
title = "Cotton-wool-like bioactive glasses for bone regeneration",
abstract = "Inorganic sol–gel solutions were electrospun to produce the first bioactive three-dimensional (3-D) scaffolds for bone tissue regeneration with a structure like cotton-wool (or cotton candy). This flexible 3-D fibrous structure is ideal for packing into complex defects. It also has large inter-fiber spaces to promote vascularization, penetration of cells and transport of nutrients throughout the scaffold. The 3-D fibrous structure was obtained by electrospinning, where the applied electric field and the instabilities exert tremendous force on the spinning jet, which is required to be viscoelastic to prevent jet break up. Previously, polymer binding agents were used with inorganic solutions to produce electrospun composite two-dimensional fibermats, requiring calcination to remove the polymer. This study presents novel reaction and processing conditions for producing a viscoelastic inorganic sol–gel solution that results in fibers by the entanglement of the intermolecularly overlapped nanosilica species in the solution, eliminating the need for a binder. Three-dimensional cotton-wool-like structures were only produced when solutions containing calcium nitrate were used, suggesting that the charge of the Ca2+ ions had a significant effect. The resulting bioactive silica fibers had a narrow diameter range of 0.5–2 μm and were nanoporous. A hydroxycarbonate apatite layer was formed on the fibers within the first 12 h of soaking in simulated body fluid. MC3T3-E1 preosteoblast cells cultured on the fibers showed no adverse cytotoxic effect and they were observed to attach to and spread in the material.",
keywords = "electrospinning, Sol-gel, bone regeneration scaffold, 3-D cotton-wool-like structure, inorganic fibers",
author = "Gowsihan Poologasundarampillai and D. Wang and S. Li and J. Nakamura and R. Bradley and Lee, {P. D.} and Stevens, {M. M.} and McPhail, {David S.} and Toshihiro Kasuga and Jones, {J. R.}",
year = "2014",
month = "8",
day = "1",
doi = "10.1016/j.actbio.2014.05.020",
language = "English",
volume = "10",
pages = "3733--3746",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier Doyma",
number = "8",

}

RIS

TY - JOUR

T1 - Cotton-wool-like bioactive glasses for bone regeneration

AU - Poologasundarampillai, Gowsihan

AU - Wang, D.

AU - Li, S.

AU - Nakamura, J.

AU - Bradley, R.

AU - Lee, P. D.

AU - Stevens, M. M.

AU - McPhail, David S.

AU - Kasuga, Toshihiro

AU - Jones, J. R.

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Inorganic sol–gel solutions were electrospun to produce the first bioactive three-dimensional (3-D) scaffolds for bone tissue regeneration with a structure like cotton-wool (or cotton candy). This flexible 3-D fibrous structure is ideal for packing into complex defects. It also has large inter-fiber spaces to promote vascularization, penetration of cells and transport of nutrients throughout the scaffold. The 3-D fibrous structure was obtained by electrospinning, where the applied electric field and the instabilities exert tremendous force on the spinning jet, which is required to be viscoelastic to prevent jet break up. Previously, polymer binding agents were used with inorganic solutions to produce electrospun composite two-dimensional fibermats, requiring calcination to remove the polymer. This study presents novel reaction and processing conditions for producing a viscoelastic inorganic sol–gel solution that results in fibers by the entanglement of the intermolecularly overlapped nanosilica species in the solution, eliminating the need for a binder. Three-dimensional cotton-wool-like structures were only produced when solutions containing calcium nitrate were used, suggesting that the charge of the Ca2+ ions had a significant effect. The resulting bioactive silica fibers had a narrow diameter range of 0.5–2 μm and were nanoporous. A hydroxycarbonate apatite layer was formed on the fibers within the first 12 h of soaking in simulated body fluid. MC3T3-E1 preosteoblast cells cultured on the fibers showed no adverse cytotoxic effect and they were observed to attach to and spread in the material.

AB - Inorganic sol–gel solutions were electrospun to produce the first bioactive three-dimensional (3-D) scaffolds for bone tissue regeneration with a structure like cotton-wool (or cotton candy). This flexible 3-D fibrous structure is ideal for packing into complex defects. It also has large inter-fiber spaces to promote vascularization, penetration of cells and transport of nutrients throughout the scaffold. The 3-D fibrous structure was obtained by electrospinning, where the applied electric field and the instabilities exert tremendous force on the spinning jet, which is required to be viscoelastic to prevent jet break up. Previously, polymer binding agents were used with inorganic solutions to produce electrospun composite two-dimensional fibermats, requiring calcination to remove the polymer. This study presents novel reaction and processing conditions for producing a viscoelastic inorganic sol–gel solution that results in fibers by the entanglement of the intermolecularly overlapped nanosilica species in the solution, eliminating the need for a binder. Three-dimensional cotton-wool-like structures were only produced when solutions containing calcium nitrate were used, suggesting that the charge of the Ca2+ ions had a significant effect. The resulting bioactive silica fibers had a narrow diameter range of 0.5–2 μm and were nanoporous. A hydroxycarbonate apatite layer was formed on the fibers within the first 12 h of soaking in simulated body fluid. MC3T3-E1 preosteoblast cells cultured on the fibers showed no adverse cytotoxic effect and they were observed to attach to and spread in the material.

KW - electrospinning

KW - Sol-gel

KW - bone regeneration scaffold

KW - 3-D cotton-wool-like structure

KW - inorganic fibers

U2 - 10.1016/j.actbio.2014.05.020

DO - 10.1016/j.actbio.2014.05.020

M3 - Article

VL - 10

SP - 3733

EP - 3746

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 8

ER -