TY - JOUR
T1 - Cooperativity between KorB and TrbA repressors of broad host range plasmid RK2
AU - Zatyka, Malgorzata
AU - Bingle, Lewis
AU - Jones, Antony
AU - Thomas, Christopher
PY - 2001/2/1
Y1 - 2001/2/1
N2 - The KorB and TrbA proteins of broad-host-range plasmid RK2 are key regulators of the plasmid genes required for conjugative transfer. trbBp is the primary promoter responsible for expression of mating pair formation genes, We show that despite the targets for KorB and TrbA at trbBp being about 165 bp apart, 189 bp upstream of the transcription start point and overlapping the -10 region, respectively, these two proteins show up to 10-fold cooperativity for the repression of trbBp, Deletion analysis of TrbA showed that the C-terminal domain (CTD), which has a high degree of sequence conservation with the CTD of KorA, is required for this cooperativity: with KorB, Western blotting demonstrated that the apparently mutual enhancement of repression is not due simply to elevation of repressor level by the presence of the second protein, suggesting that the basis for cooperativity is interaction between KorB and TrbA bound at their respective operators.
AB - The KorB and TrbA proteins of broad-host-range plasmid RK2 are key regulators of the plasmid genes required for conjugative transfer. trbBp is the primary promoter responsible for expression of mating pair formation genes, We show that despite the targets for KorB and TrbA at trbBp being about 165 bp apart, 189 bp upstream of the transcription start point and overlapping the -10 region, respectively, these two proteins show up to 10-fold cooperativity for the repression of trbBp, Deletion analysis of TrbA showed that the C-terminal domain (CTD), which has a high degree of sequence conservation with the CTD of KorA, is required for this cooperativity: with KorB, Western blotting demonstrated that the apparently mutual enhancement of repression is not due simply to elevation of repressor level by the presence of the second protein, suggesting that the basis for cooperativity is interaction between KorB and TrbA bound at their respective operators.
UR - http://www.scopus.com/inward/record.url?scp=0035155185&partnerID=8YFLogxK
U2 - 10.1128/JB.183.3.1022-1031.2001
DO - 10.1128/JB.183.3.1022-1031.2001
M3 - Article
C2 - 11208801
VL - 183
SP - 1022
EP - 1031
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 3
ER -