Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

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Authors

  • Eliza Ploskon
  • Sara C. Wagner
  • Andrew D. Ellington
  • Michael C. Jewett
  • Paula J. Booth

Colleges, School and Institutes

Abstract

DNA–protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein–protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA–protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.

Details

Original languageEnglish
Pages (from-to)427-434
Number of pages8
JournalBioconjugate Chemistry
Volume26
Issue number3
Publication statusPublished - 20 Jan 2015