Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Research output: Contribution to journalArticlepeer-review


  • Eliza Ploskon
  • Sara C. Wagner
  • Andrew D. Ellington
  • Michael C. Jewett
  • Paula J. Booth

Colleges, School and Institutes


DNA–protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein–protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA–protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.


Original languageEnglish
Pages (from-to)427-434
Number of pages8
JournalBioconjugate Chemistry
Issue number3
Publication statusPublished - 20 Jan 2015