TY - JOUR
T1 - Contributions to our understanding of T cell physiology through unveiling the T cell proteome
AU - Grant, Melissa
AU - Scheel-Toellner, Dagmar
AU - Griffiths, Helen
PY - 2007/7/1
Y1 - 2007/7/1
N2 - Since the sequencing of the human genome was completed, attention has turned to examining the functionality of the molecular machinery, in particular of protein expression. Differential proteome analysis by two-dimensional electrophoresis has been adopted to study changes in T cell proteomes during T cell activation, and this work is increasing our understanding of the complexity of signals elicited across multiple pathways. The purpose of this review is to summarize the available evidence in the application of proteomic techniques and methodologies to understand T cell receptor activation from lipid raft and cytoskeletal rearrangements, through to signalling cascades, transcription factor modulation and changes in protein expression patterns. These include post-translational modifications, which are not encoded by the genome.
AB - Since the sequencing of the human genome was completed, attention has turned to examining the functionality of the molecular machinery, in particular of protein expression. Differential proteome analysis by two-dimensional electrophoresis has been adopted to study changes in T cell proteomes during T cell activation, and this work is increasing our understanding of the complexity of signals elicited across multiple pathways. The purpose of this review is to summarize the available evidence in the application of proteomic techniques and methodologies to understand T cell receptor activation from lipid raft and cytoskeletal rearrangements, through to signalling cascades, transcription factor modulation and changes in protein expression patterns. These include post-translational modifications, which are not encoded by the genome.
KW - T cells
KW - proteomics
KW - 2D-PAGE
U2 - 10.1111/j.1365-2249.2007.03395.x
DO - 10.1111/j.1365-2249.2007.03395.x
M3 - Review article
C2 - 17488298
SN - 1365-2249
VL - 149
SP - 9
EP - 15
JO - Clinical & Experimental Immunology
JF - Clinical & Experimental Immunology
IS - 1
ER -