Consequences of cathepsin C inactivation for membrane exposure of proteinase 3, the target antigen in autoimmune vasculitis

Research output: Contribution to journalArticlepeer-review

Authors

  • Seda Seren
  • Maha Rashed Abouzaid
  • Claudia Eulenberg-Gustavus
  • Hala Nasr Soliman
  • Uwe Jerke
  • Koffi N'Guessan
  • Sandrine Dallet-Choisy
  • Adam Lesner
  • Conni Lauritzen
  • Beate Schacher
  • Peter Eickholz
  • Nikoletta Nagy
  • Marta Szell
  • Cécile Croix
  • Marie-Claude Viaud-Massuard
  • Abdullah Al Farraj Aldosari
  • Shivanna Ragunatha
  • Mostafa Ibrahim Mostafa
  • Francesca Giampieri
  • Maurizio Battino
  • Hélène Cornillier
  • Gérard Lorette
  • Jean-Louis Stephan
  • Cyril Goizet
  • John Pedersen
  • Francis Gauthier
  • Dieter E. Jenne
  • Sylvain Marchand-Adam
  • Ralph Kettritz
  • Brice Korkmaz

Colleges, School and Institutes

External organisations

  • Centre for the Study of Respiratory Pathologies, University of Tours
  • Medical Molecular Genetics, National Research Centre, Cairo 12622, Egypt.
  • Faculty of Chemistry, University of Gdansk
  • Unizyme Laboratories A/S
  • Department of Periodontology, Center for Oral Medicine, Goethe-University Frankfurt
  • Department of Medical Genetics, University of Szeged
  • Genetics, Immunotherapy, Chemistry and Cancer, University of Tours
  • Department of Prosthetic Dental Science, College of Dentistry, King Saud University
  • Department of Dermatology, Venereology, and Leprosy, ESIC Medical College and PGIMSR Rajajinagar
  • Department of Clinical Sciences, School of Medicine, Universitá Politecnica delle Marche
  • Service de Dermatologie, Centre Hospitalier Universitaire de Tours, Université de Tours
  • Division of Nephrology and Intensive Care Medicine, Medical Department, Charité-Universitätsmedizin
  • Comprehensive Pneumology Center, Institute of Lung Biology and Disease, German Center for Lung Research (DZL)

Abstract

Membrane-bound proteinase 3 (PR3m) is the main target antigen of anti-neutrophil cytoplasmic autoantibodies (ANCA) in granulomatosis with polyangiitis, a systemic small-vessel vasculitis. Binding of ANCA to PR3m triggers neutrophil activation with the secretion of enzymatically active PR3 and related neutrophil serine proteases, thereby contributing to vascular damage. PR3 and related proteases are activated from pro-forms by the lysosomal cysteine protease cathepsin C (CatC) during neutrophil maturation. We hypothesized that pharmacological inhibition of CatC provides an effective measure to reduce PR3m and therefore has implications as a novel therapeutic approach in granulomatosis with polyangiitis. We first studied neutrophilic PR3 from 24 patients with Papillon-Lefèvre syndrome (PLS), a genetic form of CatC deficiency. PLS neutrophil lysates showed a largely reduced but still detectable (0.5-4%) PR3 activity when compared with healthy control cells. Despite extremely low levels of cellular PR3, the amount of constitutive PR3m expressed on the surface of quiescent neutrophils and the typical bimodal membrane distribution pattern were similar to what was observed in healthy neutrophils. However, following cell activation, there was no significant increase in the total amount of PR3m on PLS neutrophils, whereas the total amount of PR3m on healthy neutrophils was significantly increased. We then explored the effect of pharmacological CatC inhibition on PR3 stability in normal neutrophils using a potent cell-permeable CatC inhibitor and a CD34+ hematopoietic stem cell model. Human CD34+ hematopoietic stem cells were treated with the inhibitor during neutrophil differentiation over 10 days. We observed strong reductions in PR3m, cellular PR3 protein, and proteolytic PR3 activity, whereas neutrophil differentiation was not compromised.

Details

Original languageEnglish
Pages (from-to)12415-12428
Number of pages14
JournalJournal of Biological Chemistry
Volume293
Issue number32
Early online date20 Jun 2018
Publication statusPublished - 10 Aug 2018