Connecting color with assembly in the fluorescent B-phycoerythrin protein complex

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

External organisations

  • Utrecht University

Abstract

Phycoerythrin is the major light-harvesting pigment protein in red algae and is nowadays widely used as a fluorescent probe in biotechnological applications such as flow cytometry and immunofluorescence microscopy. In addition, it has had substantial economic impact due to its potential as a natural food colorant. However, knowledge on the precise molecular composition of phycoerythrin is limited. Here, we use a combination of high-resolution native mass spectrometry (MS) and fluorescence spectroscopy to characterize the assembly properties of the B-phycoerythrin protein complex from Porphyridium cruentum. Our data highlight the stabilizing role of the γ subunit in the intact B-phycoerythrin protein complex. In addition, by native MS we monitor B-phycoerythrin (dis)assembly intermediates, providing insight into which species contribute to B-phycoerythrins color and the factors that give B-phycoerythrin its highly fluorescent properties. Together, the data provide significant insights into the structural properties of B-phycoerythrin which is beneficial for its use within the biotechnology industry.

Details

Original languageEnglish
Pages (from-to)178-187
Number of pages10
JournalFEBS Journal
Volume285
Issue number1
Publication statusPublished - 1 Jan 2018

Keywords

  • fluorescent probes, light-harvesting complex, native mass spectrometry, phycobiliproteins, phycoerythrin