Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042

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Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042. / Chaudhuri, RR; Sebaihia, M; Hobman, JL; Webber, Mark; Leyton, Denisse; Goldberg, Martin; Cunningham, Adam; Scott-Tucker, A; Ferguson, Paul; Thomas, Christopher; Frankel, G; Tang, CM; Dudley, EG; Roberts, IS; Rasko, DA; Pallen, Mark; Parkhill, J; Nataro, JP; Thomson, NR; Henderson, Ian.

In: PLoS ONE, Vol. 5, No. 1, e8801, 01.01.2010.

Research output: Contribution to journalArticlepeer-review

Harvard

Chaudhuri, RR, Sebaihia, M, Hobman, JL, Webber, M, Leyton, D, Goldberg, M, Cunningham, A, Scott-Tucker, A, Ferguson, P, Thomas, C, Frankel, G, Tang, CM, Dudley, EG, Roberts, IS, Rasko, DA, Pallen, M, Parkhill, J, Nataro, JP, Thomson, NR & Henderson, I 2010, 'Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042', PLoS ONE, vol. 5, no. 1, e8801. https://doi.org/10.1371/journal.pone.0008801

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Chaudhuri, RR ; Sebaihia, M ; Hobman, JL ; Webber, Mark ; Leyton, Denisse ; Goldberg, Martin ; Cunningham, Adam ; Scott-Tucker, A ; Ferguson, Paul ; Thomas, Christopher ; Frankel, G ; Tang, CM ; Dudley, EG ; Roberts, IS ; Rasko, DA ; Pallen, Mark ; Parkhill, J ; Nataro, JP ; Thomson, NR ; Henderson, Ian. / Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042. In: PLoS ONE. 2010 ; Vol. 5, No. 1.

Bibtex

@article{11e915c28bd14d40991ed5b56eb1c6ce,
title = "Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042",
abstract = "Background Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog{\texttrademark} Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.",
author = "RR Chaudhuri and M Sebaihia and JL Hobman and Mark Webber and Denisse Leyton and Martin Goldberg and Adam Cunningham and A Scott-Tucker and Paul Ferguson and Christopher Thomas and G Frankel and CM Tang and EG Dudley and IS Roberts and DA Rasko and Mark Pallen and J Parkhill and JP Nataro and NR Thomson and Ian Henderson",
note = "Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042",
year = "2010",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0008801",
language = "English",
volume = "5",
journal = "PLoSONE",
issn = "1932-6203",
publisher = "Public Library of Science (PLOS)",
number = "1",

}

RIS

TY - JOUR

T1 - Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042

AU - Chaudhuri, RR

AU - Sebaihia, M

AU - Hobman, JL

AU - Webber, Mark

AU - Leyton, Denisse

AU - Goldberg, Martin

AU - Cunningham, Adam

AU - Scott-Tucker, A

AU - Ferguson, Paul

AU - Thomas, Christopher

AU - Frankel, G

AU - Tang, CM

AU - Dudley, EG

AU - Roberts, IS

AU - Rasko, DA

AU - Pallen, Mark

AU - Parkhill, J

AU - Nataro, JP

AU - Thomson, NR

AU - Henderson, Ian

N1 - Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Background Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

AB - Background Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

U2 - 10.1371/journal.pone.0008801

DO - 10.1371/journal.pone.0008801

M3 - Article

C2 - 20098708

VL - 5

JO - PLoSONE

JF - PLoSONE

SN - 1932-6203

IS - 1

M1 - e8801

ER -