Comparison of GC-MS, HPLC-MS and SIFT-MS in conjunction with multivariate classification for the diagnosis of Crohn's disease in urine

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Comparison of GC-MS, HPLC-MS and SIFT-MS in conjunction with multivariate classification for the diagnosis of Crohn's disease in urine. / Cauchi, M.; Fowler, D. P.; Walton, C.; Turner, C.; Waring, R. H.; Ramsden, D. B.; Hunter, J. O.; Teale, P.; Cole, J. A.; Bessant, C.

In: Analytical Methods, Vol. 7, No. 19, 07.10.2015, p. 8379-8385.

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Cauchi, M. ; Fowler, D. P. ; Walton, C. ; Turner, C. ; Waring, R. H. ; Ramsden, D. B. ; Hunter, J. O. ; Teale, P. ; Cole, J. A. ; Bessant, C. / Comparison of GC-MS, HPLC-MS and SIFT-MS in conjunction with multivariate classification for the diagnosis of Crohn's disease in urine. In: Analytical Methods. 2015 ; Vol. 7, No. 19. pp. 8379-8385.

Bibtex

@article{461b23e633de481f902480cea9314885,
title = "Comparison of GC-MS, HPLC-MS and SIFT-MS in conjunction with multivariate classification for the diagnosis of Crohn's disease in urine",
abstract = "The developed world has seen an alarming increase in the incidence of gastrointestinal diseases, among the most common of which is Crohn's disease (CD) in the young. The current “gold standard” techniques for diagnosis are often costly, time consuming, inefficient, invasive, and offer poor sensitivities and specificities. This paper compares the performances of three hyphenated instrumental techniques that have been suggested as rapid methods for the non-invasive diagnosis of CD from urine. These techniques are gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Each of these techniques is followed by multivariate classification to provide a diagnosis based on the acquired data. The most promising results for potentially diagnosing CD was via HPLC-MS. An overall classification accuracy of 73% (74% specificity; 73% sensitivity) was achieved for differentiating CD from healthy controls, statistically significant at 95% confidence.",
author = "M. Cauchi and Fowler, {D. P.} and C. Walton and C. Turner and Waring, {R. H.} and Ramsden, {D. B.} and Hunter, {J. O.} and P. Teale and Cole, {J. A.} and C. Bessant",
year = "2015",
month = oct,
day = "7",
doi = "10.1039/C5AY01322D",
language = "English",
volume = "7",
pages = "8379--8385",
journal = "Analytical Methods",
issn = "1759-9660",
publisher = "Royal Society of Chemistry",
number = "19",

}

RIS

TY - JOUR

T1 - Comparison of GC-MS, HPLC-MS and SIFT-MS in conjunction with multivariate classification for the diagnosis of Crohn's disease in urine

AU - Cauchi, M.

AU - Fowler, D. P.

AU - Walton, C.

AU - Turner, C.

AU - Waring, R. H.

AU - Ramsden, D. B.

AU - Hunter, J. O.

AU - Teale, P.

AU - Cole, J. A.

AU - Bessant, C.

PY - 2015/10/7

Y1 - 2015/10/7

N2 - The developed world has seen an alarming increase in the incidence of gastrointestinal diseases, among the most common of which is Crohn's disease (CD) in the young. The current “gold standard” techniques for diagnosis are often costly, time consuming, inefficient, invasive, and offer poor sensitivities and specificities. This paper compares the performances of three hyphenated instrumental techniques that have been suggested as rapid methods for the non-invasive diagnosis of CD from urine. These techniques are gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Each of these techniques is followed by multivariate classification to provide a diagnosis based on the acquired data. The most promising results for potentially diagnosing CD was via HPLC-MS. An overall classification accuracy of 73% (74% specificity; 73% sensitivity) was achieved for differentiating CD from healthy controls, statistically significant at 95% confidence.

AB - The developed world has seen an alarming increase in the incidence of gastrointestinal diseases, among the most common of which is Crohn's disease (CD) in the young. The current “gold standard” techniques for diagnosis are often costly, time consuming, inefficient, invasive, and offer poor sensitivities and specificities. This paper compares the performances of three hyphenated instrumental techniques that have been suggested as rapid methods for the non-invasive diagnosis of CD from urine. These techniques are gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Each of these techniques is followed by multivariate classification to provide a diagnosis based on the acquired data. The most promising results for potentially diagnosing CD was via HPLC-MS. An overall classification accuracy of 73% (74% specificity; 73% sensitivity) was achieved for differentiating CD from healthy controls, statistically significant at 95% confidence.

U2 - 10.1039/C5AY01322D

DO - 10.1039/C5AY01322D

M3 - Article

VL - 7

SP - 8379

EP - 8385

JO - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

IS - 19

ER -