Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster

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Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster. / Flint, J; Tufarelli, C; Peden, J; Clark, K; Daniels, R; Hardison, R; Miller, W; Philipsen, S; Tan-Un, K.C; McMorrow, T; Frampton, Jonathan; Alter, B; Frischauf, A-M; Higgs, DR.

In: Human Molecular Genetics, Vol. 10, 01.02.2001, p. 371-382.

Research output: Contribution to journalArticle

Harvard

Flint, J, Tufarelli, C, Peden, J, Clark, K, Daniels, R, Hardison, R, Miller, W, Philipsen, S, Tan-Un, KC, McMorrow, T, Frampton, J, Alter, B, Frischauf, A-M & Higgs, DR 2001, 'Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster', Human Molecular Genetics, vol. 10, pp. 371-382. https://doi.org/10.1093/hmg/10.4.371

APA

Flint, J., Tufarelli, C., Peden, J., Clark, K., Daniels, R., Hardison, R., Miller, W., Philipsen, S., Tan-Un, K. C., McMorrow, T., Frampton, J., Alter, B., Frischauf, A-M., & Higgs, DR. (2001). Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster. Human Molecular Genetics, 10, 371-382. https://doi.org/10.1093/hmg/10.4.371

Vancouver

Author

Flint, J ; Tufarelli, C ; Peden, J ; Clark, K ; Daniels, R ; Hardison, R ; Miller, W ; Philipsen, S ; Tan-Un, K.C ; McMorrow, T ; Frampton, Jonathan ; Alter, B ; Frischauf, A-M ; Higgs, DR. / Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster. In: Human Molecular Genetics. 2001 ; Vol. 10. pp. 371-382.

Bibtex

@article{6bfb571992284e9f9e9463d7520a8874,
title = "Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster",
abstract = "We have cloned, sequenced and annotated segments of DNA spanning the mouse, chicken and pufferfish alpha globin gene clusters and compared them with the corresponding region in man. This has defined a small segment ( approximately 135-155 kb) of synteny and conserved gene order, which may contain all of the elements required to fully regulate alpha globin gene expression from its natural chromosomal environment. Comparing human and mouse sequences using previously described methods failed to identify the known regulatory elements. However, refining these methods by ranking identity scores of non-coding sequences, we found conserved sequences including the previously characterized alpha globin major regulatory element. In chicken and pufferfish, regions that may correspond to this element were found by analysing the distribution of transcription factor binding sites. Regions identified in this way act as strong enhancer elements in expression assays. In addition to delimiting the alpha globin chromosomal domain, this study has enabled us to develop a more sensitive and accurate routine for identifying regulatory elements in the human genome.",
author = "J Flint and C Tufarelli and J Peden and K Clark and R Daniels and R Hardison and W Miller and S Philipsen and K.C Tan-Un and T McMorrow and Jonathan Frampton and B Alter and A-M Frischauf and DR Higgs",
year = "2001",
month = feb,
day = "1",
doi = "10.1093/hmg/10.4.371",
language = "English",
volume = "10",
pages = "371--382",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Comparative gene analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster

AU - Flint, J

AU - Tufarelli, C

AU - Peden, J

AU - Clark, K

AU - Daniels, R

AU - Hardison, R

AU - Miller, W

AU - Philipsen, S

AU - Tan-Un, K.C

AU - McMorrow, T

AU - Frampton, Jonathan

AU - Alter, B

AU - Frischauf, A-M

AU - Higgs, DR

PY - 2001/2/1

Y1 - 2001/2/1

N2 - We have cloned, sequenced and annotated segments of DNA spanning the mouse, chicken and pufferfish alpha globin gene clusters and compared them with the corresponding region in man. This has defined a small segment ( approximately 135-155 kb) of synteny and conserved gene order, which may contain all of the elements required to fully regulate alpha globin gene expression from its natural chromosomal environment. Comparing human and mouse sequences using previously described methods failed to identify the known regulatory elements. However, refining these methods by ranking identity scores of non-coding sequences, we found conserved sequences including the previously characterized alpha globin major regulatory element. In chicken and pufferfish, regions that may correspond to this element were found by analysing the distribution of transcription factor binding sites. Regions identified in this way act as strong enhancer elements in expression assays. In addition to delimiting the alpha globin chromosomal domain, this study has enabled us to develop a more sensitive and accurate routine for identifying regulatory elements in the human genome.

AB - We have cloned, sequenced and annotated segments of DNA spanning the mouse, chicken and pufferfish alpha globin gene clusters and compared them with the corresponding region in man. This has defined a small segment ( approximately 135-155 kb) of synteny and conserved gene order, which may contain all of the elements required to fully regulate alpha globin gene expression from its natural chromosomal environment. Comparing human and mouse sequences using previously described methods failed to identify the known regulatory elements. However, refining these methods by ranking identity scores of non-coding sequences, we found conserved sequences including the previously characterized alpha globin major regulatory element. In chicken and pufferfish, regions that may correspond to this element were found by analysing the distribution of transcription factor binding sites. Regions identified in this way act as strong enhancer elements in expression assays. In addition to delimiting the alpha globin chromosomal domain, this study has enabled us to develop a more sensitive and accurate routine for identifying regulatory elements in the human genome.

U2 - 10.1093/hmg/10.4.371

DO - 10.1093/hmg/10.4.371

M3 - Article

C2 - 11157800

VL - 10

SP - 371

EP - 382

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

ER -